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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of bovine papillomavirus DNA in sarcoid-affected and healthy free-roaming zebra (Equus zebra) populations in South Africa
Journal of Virological Methods, Volume 158, No. 1-2, Year 2009
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Description
The endangered Cape mountain zebra (Equus zebra zebra) is protected in small numbers in a few isolated populations in South African game parks. Since 1995, sarcoid lesions appeared in zebras in two of the parks. This study was undertaken to investigate if bovine papillomavirus (BPV) is associated with sarcoids in these zebras. A conventional PCR, targeting the E5 ORF of BPV, and subsequent RFLP analysis were initially used to demonstrate the presence of BPV-1 and -2 DNAs in zebra sarcoid tumours. A rapid, sensitive and reliable real-time PCR to detect and distinguish between BPV-1 and -2 infections in zebras was developed. With this assay it was demonstrated that BPV-1 and -2 DNA (either single or mixed infections) are present in sarcoid tumour, healthy skin and blood of sarcoid-affected and healthy zebras from sarcoid-affected parks as well as in the blood of zebras from parks where no sarcoid has been observed before. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
van Dyk, E.
South Africa, Pretoria
University of Pretoria
Oosthuizen, Marinda C.
South Africa, Pretoria
University of Pretoria
Bosman, Anna Marì
South Africa, Pretoria
University of Pretoria
Nel, Pierre J.
Unknown Affiliation
Zimmerman, David
South Africa, Pretoria
South African National Parks
Venter, Estelle Hildegard
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 39
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.jviromet.2009.02.008
ISSN:
01660934
Research Areas
Cancer
Genetics And Genomics
Study Locations
South Africa