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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
In-situ generation of differential sensors that fingerprint kinases and the cellular response to their expression
Journal of the American Chemical Society, Volume 135, No. 39, Year 2013
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Description
Mitogen-activated protein (MAP) kinases are responsible for many cellular functions, and their malfunction manifests itself in several human diseases. Usually, monitoring the phosphorylation states of MAP kinases in vitro requires the preparation and purification of the proteins or Western blotting. Herein, we report an array sensing approach for the differentiation of MAP kinases and their phosphorylated counterparts in vitro. This technique utilizes a library of differential receptors created in situ containing peptides known for affinity to MAP kinases, and a Zn(II)-dipicolylamine complex that binds phosphate groups on proteins. An indicator-displacement assay signals the binding of the individual receptors to the kinases, while chemometrics is used to create a fingerprint for the kinases and their state of activity. For example, linear discriminant analysis correctly identified kinase activity with a classification accuracy of 97.5% in vitro, while the cellular response to kinase expression was classified with 100% accuracy. © 2013 American Chemical Society.
Authors & Co-Authors
Zamora-Olivares, Diana P.
United States, Austin
The University of Texas at Austin
Kaoud, Tamer S.
United States, Austin
The University of Texas at Austin
Egypt, Minya
Faculty of Pharmacy
Dalby, Kevin N.
United States, Austin
The University of Texas at Austin
Anslyn, Eric V.
United States, Austin
The University of Texas at Austin
Statistics
Citations: 59
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1021/ja407397z
ISSN:
00027863
e-ISSN:
15205126