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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN
Virology, Volume 446, No. 1-2, Year 2013
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Description
We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN. © 2013 Elsevier Inc.
Authors & Co-Authors
Alexandre, Kabamba Bankoledi
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
University of the Witwatersrand
Moore, Penny L.
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
University of the Witwatersrand
Nonyane, Molati
South Africa, Johannesburg
National Institute for Communicable Diseases
Gray, Elin Solomonovna
South Africa, Johannesburg
National Institute for Communicable Diseases
Ranchobe, Nthabeleng
South Africa, Johannesburg
National Institute for Communicable Diseases
Chakauya, Ereck
South Africa, Pretoria
The Council for Scientific and Industrial Research
McMahon, James B.
United States, Frederick
National Cancer Institute at Frederick
O'Keefe, Barry R.
United States, Frederick
National Cancer Institute at Frederick
Chikwamba, Rachel K.
South Africa, Pretoria
The Council for Scientific and Industrial Research
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
University of the Witwatersrand
Statistics
Citations: 25
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.virol.2013.07.019
ISSN:
00426822
e-ISSN:
10960341
Research Areas
Infectious Diseases