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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Gene expression profiling of single bovine embryos uncovers significant effectsof in vitro maturation, fertilization and culture
Molecular Reproduction and Development, Volume 76, No. 1, Year 2009
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Description
In vitro production (IVP) has been shown to affect embryonic gene expression and often result in large offspring syndrome (LOS) in cattle and sheep. To dissect the effects of in vitro maturation, fertilization and culture on bovine embryos, we compared the expression profiles of single blastocysts generated by: (1) in vitro maturation, fertilization and culture (IVF); (2) in vivo maturation, fertilization and in vitro culture (IVD); and (3) in vivo maturation, fertilization and development (AI). To conduct expression profiling, total RNA was isolated from individual embryos, linearly amplified and hybridized to a custom bovine cDNA microarray containing approximately 6,300 unique genes. There were 306, 367, and 200 genes differentially expressed between the AI and IVD, IVF and IVD, and AI and IVF comparisons, respectively. Interestingly, 44 differentially expressed genes were identified between the AI embryos and both the IVF and IVD embryos, making these potential candidates for LOS. There were 60 genes differentially expressed between the IVF embryos and the AI and IVD embryos. The Gene Ontology category "RNA processing" was over-represented among the genes that were down-regulated in the IVF embryos, indicating an effect of in vitro oocyte maturation/fertilization on the ability to transcribe maternal RNA stores. A culture effect on the expression of genes involved in translation was also observed by the comparison of AI with IVD embryos. © 2008 Wiley-Liss, Inc.
Authors & Co-Authors
Smith, Sadie L.
United States, Storrs
University of Connecticut
United States, New Haven
Yale University
Everts, Robin E.
United States, Urbana
University of Illinois Urbana-champaign
United States, San Diego
Sequenom Inc.
Sung, Li Ying
United States, Storrs
University of Connecticut
Taiwan, Taipei
National Taiwan University
Du, Fuliang
United States, Storrs
University of Connecticut
Page, Raymond L.
United States, Worcester
Worcester Polytechnic Institute
Henderson, Boyd
United States, Elizabethtown
Henderson Veterinary Associates
Rodríguez-Zas, Sandra L.
United States, Urbana
University of Illinois Urbana-champaign
Nedambale, Tshimangadzo Lucky
United States, Storrs
University of Connecticut
South Africa, Pretoria
Agricultural Research Council, Pretoria
Renard, Jean Paul
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Lewin, Harris A.
United States, Urbana
University of Illinois Urbana-champaign
Yang, Xiangzhong Jerry
United States, Storrs
University of Connecticut
South Africa, Pretoria
Agricultural Research Council, Pretoria
Tian, Xiuchun (Cindy)
United States, Storrs
University of Connecticut
Statistics
Citations: 69
Authors: 12
Affiliations: 9
Identifiers
Doi:
10.1002/mrd.20927
ISSN:
1040452X
e-ISSN:
10982795
Research Areas
Genetics And Genomics
Maternal And Child Health