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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Rotavirus genetic diversity, disease association, and temporal change in hospitalized rural kenyan children
Journal of Infectious Diseases, Volume 202, No. SUPPL. 1, Year 2010
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Description
Background. The effectiveness of rotavirus vaccines will be dependent on the immunity conferred against prevalent and emergent variants causing severe diarrheal disease. Longitudinal surveillance of disease-causing strains is a prerequisite to intervention. Methods. Molecular characterization was conducted on rotavirus-positive stool samples from children admitted with diarrhea to a rural district hospital during 2002-2004. Extracted viral RNA was separated by polyacrylamide gel electrophoresis, and rotavirus VP4 (P types) and VP7 (G types) specificities were determined. Results. Among 558 investigated cases, the predominant genotype was P[8]G1 (42%), followed by P[8]G9 (15%), P[4]G8 (7%), P[6]G8 (6%), and P[8]G8 (4%), with 10% mixed strains. Overall, there were 6 different P types and 7 G types. No association was identified between genotype and child age, sex, or severity of diarrhea. The P and G genotypes and polyacrylamide gel electropherotypes showed significant temporal variation in frequency: P[8]G1 decreased from 51% (95% confidence interval [CI], 43%-58%) in 2002 to 30% (95% CI, 24%-37%) in 2004, and P[4]G8 increased from 2% (95% CI, 0%-5%) in 2002 to 13% (95% CI, 9%-19%). Quarterly data revealed seasonally endemic and emergence and/or decay patterns. Conclusions. Our study of rotavirus strains causing severe diarrhea in rural Kenyan children showed a predominance of P[8]G1 and confirms the importance of G8 and G9 strains in sub-Saharan Africa. Considerable genetic diversity of rotavirus strains was observed, including substantial mixed and unusual types, coupled with significant temporal strain variation and emergence. These results warn of variable vaccine efficacy and the need for long-term surveillance of circulating rotavirus genotypes. © 2010 by the Infectious Diseases Society of America.
Authors & Co-Authors
Nokes, David James
Kenya, Kilifi
Centre for Geographic Medicine Research
United Kingdom, Coventry
University of Warwick
Peenze, Ina
United Kingdom, London
Medical Research Council
Netshifhefhe, Lufuno
United Kingdom, London
Medical Research Council
Abwao, John
Kenya, Kilifi
Centre for Geographic Medicine Research
de Beer, Mariet C.
United Kingdom, London
Medical Research Council
Seheri, Luyanda Mapaseka
United Kingdom, London
Medical Research Council
Williams, Thomas Neil
Kenya, Kilifi
Centre for Geographic Medicine Research
United Kingdom, Oxford
University of Oxford Medical Sciences Division
United Kingdom, Oxford
Nuffield Department of Medicine
Ghana, Accra
Indepth Network
Page, Nicola A.
United Kingdom, London
Medical Research Council
South Africa, Johannesburg
National Institute for Communicable Diseases
Steele, Duncan A.
United Kingdom, London
Medical Research Council
United States, Seattle
Path Seattle
Statistics
Citations: 9
Authors: 9
Affiliations: 8
Identifiers
Doi:
10.1086/653566
ISSN:
00221899
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health
Study Design
Randomised Control Trial
Cohort Study