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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
N-linked glycan structures and their expressions change in the blood sera of ovarian cancer patients
Journal of Proteome Research, Volume 11, No. 4, Year 2012
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Description
Glycosylated proteins play important roles in a broad spectrum of biochemical and biological processes, and prior reports have suggested that changes in protein glycosylation occur during cancer initiation and progression. Ovarian cancer (OC) is a fatal malignancy, most commonly diagnosed after the development of metastases. Therefore, early detection of OC is key to improving survival. To this end, specific changes of the serum glycome have been proposed as possible biomarkers for different types of cancers. In this study, we extend this concept to OC. To characterize differences in total N-glycan levels, serum samples provided by 20 healthy control women were compared to those acquired from patients diagnosed with late-stage recurrent OC who were enrolled in an experimental treatment trial prior to receiving therapy (N = 19) and one month later, prior to the second treatment cycle (N = 11). Additionally, analyses of the N-glycans associated with IgG and characterization of the relative abundance levels of core vs outer-arm fucosylation were also performed. The N-linked glycomic profiles revealed increased abundances of tri- and tetra-branched structures with varying degrees of sialylation and fucosylation and an apparent decrease in the levels of "bisecting" glycans in OC samples compared to controls. Increased levels of a-galactosylation structures were observed on N-linked glycans derived from IgG, which were independent of the presence of fucose residues. Elevated levels of outer-arm fucosylation were also identified in the OC samples. These results allowed the control samples to be distinguished from the baseline ovarian cancer patients prior to receiving the experimental treatment. In some cases, the pre-treatment samples could be distinguished from the post-experimental treatment samples, as many of those patients showed a further progression of the disease. © 2012 American Chemical Society.
Authors & Co-Authors
Hussein, Ahmed A.A.
United States, Bloomington
Indiana University Bloomington
United States, Bloomington
National Center for Glycomics and Glycoproteomics, Bloomington
Mechref, Yehia S.
United States, Bloomington
Indiana University Bloomington
United States, Bloomington
National Center for Glycomics and Glycoproteomics, Bloomington
Novotný, Miloš V.
United States, Bloomington
Indiana University Bloomington
United States, Bloomington
National Center for Glycomics and Glycoproteomics, Bloomington
United States, Indianapolis
Indiana University School of Medicine
Statistics
Citations: 158
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1021/pr201070k
ISSN:
15353893
Research Areas
Cancer
Participants Gender
Female