Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage
Nucleic Acids Research, Volume 33, No. 17, Year 2005
Notification
URL copied to clipboard!
Description
Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52 256 transcripts per million (t.p.m.). Rare transcripts (<50 t.p.m.) were detected from 36% of genes. Sequence signatures approximated a lognormal distribution, as in microarray. Transcripts were widely distributed throughout the genome, although only 47% of 138 telomere-associated open reading frames exhibited signatures. Antisense signatures comprised 13.8% of the total, comparable with Plasmodium. Eighty five predicted genes with antisense signatures lacked a sense signature. Antisense transcripts were independently amplified from schizont cDNA and verified by sequencing. The MPSS transcripts per million for seven genes encoding schizont antigens recognized by bovine CD8 T cells varied 1000-fold. There was concordance between transcription and protein expression for heat shock proteins that were very highly expressed according to MPSS and proteomics. The data suggests a low level of baseline transcription from the majority of protein-coding genes. © The Author 2005. Published by Oxford University Press. All rights reserved.
Authors & Co-Authors
Bishop, Richard Peter
Kenya, Nairobi
International Livestock Research Institute Nairobi
Shah, Trushar M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Pellé, Roger
Kenya, Nairobi
International Livestock Research Institute Nairobi
Hoyle, David
United Kingdom, Exeter
University of Exeter
Pearson, Terry W.
Canada, Victoria
University of Victoria
Haines, Lee Rafuse
Canada, Victoria
University of Victoria
Brass, A.
United Kingdom, Manchester
The University of Manchester
Hulme, Helen
United Kingdom, Manchester
The University of Manchester
Graham, Simon P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Taracha, Evans L.N.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Kanga, Simon
United States, Rockville
J. Craig Venter Institute
Lu, Charles Q.
United States, Rockville
J. Craig Venter Institute
Hass, Brian
United States, Rockville
J. Craig Venter Institute
Wortman, Jennifer Russo
United States, Rockville
J. Craig Venter Institute
White, Owen R.
United States, Rockville
J. Craig Venter Institute
Gardner, Malcolm J.
United States, Rockville
J. Craig Venter Institute
Nene, Vishvanath M.
United States, Rockville
J. Craig Venter Institute
De Villiers, Etienne P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Statistics
Citations: 53
Authors: 18
Affiliations: 5
Identifiers
Doi:
10.1093/nar/gki818
ISSN:
03051048
e-ISSN:
13624962
Research Areas
Genetics And Genomics
Study Approach
Quantitative