Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Restoration of ovarian function after autotransplantation of intact frozen-thawed sheep ovaries with microvascular anastomosis
Fertility and Sterility, Volume 79, No. 3, Year 2003
Notification
URL copied to clipboard!
Description
Objective: To test the feasibility of transplanting an intact frozen-thawed ovary with microvascular anastomosis of the ovarian vascular pedicle to the deep inferior epigastric vessels. Design: Chronic survival study. Setting: Biological Resources Unit, The Cleveland Clinic Foundation. Animal(s): Adult merino ewes. Intervention(s): Bilateral laparoscopic oophorectomy was performed on 17 synchronized ewes. In one group of animals (Group I, n = 11), both ovaries were cryopreserved intact with their vascular pedicles. In another group of animals (Group II, n = 6), ovarian cortical strips were prepared from each ovary and cryopreserved. After thawing, follicular viability and apoptosis rates were assessed using one ovary. The other ovary was transplanted to the abdominal wall with microvascular anastomosis (Group I). In Group II, the ovarian cortical strips were placed in the anterior abdominal wall. Ovaries were harvested after 8-10 days in situ and subjected to histological evaluation. Main Outcome Measure(s): Blood flow, apoptotic signals, follicular viability, serum estradiol (E2), follicle-stimulating hormone (FSH), and histology. Result(s): No significant differences were found in the mean values of apoptosis (mostly in the atretic and some secondary follicles) and follicular viability in both groups. In Group I, immediate and long-term patency were documented in 100% and 27% (3/11) of the grafts, respectively; and postoperative FSH levels were similar to preoperative values in animals with patent vessels. In Group II, postoperative FSH levels were significantly higher than the preoperative ones (P=.03). Conclusion(s): Transplantation of an intact frozen-thawed ovary is technically feasible. Using this approach, immediate restoration of vascular supply and ovarian hormonal functions is possible. © 2003 by American Society for Reproductive Medicine.
Authors & Co-Authors
Bedaiwy, Mohamed Ali
United States, Cleveland
Cleveland Clinic Foundation
Hussein, Mahmoud Rezk Abdelwahed
United States, Cleveland
Case Western Reserve University
Biscotti, Charles V.
United States, Cleveland
Cleveland Clinic Foundation
Falcone, Tommaso
United States, Cleveland
Cleveland Clinic Foundation
Statistics
Citations: 125
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/S0015-0282(02)04842-2
ISSN:
00150282
Research Areas
Cancer
Health System And Policy
Sexual And Reproductive Health
Study Design
Randomised Control Trial