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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A genotypic approach for detection, identification, and characterization of drug resistance in Mycobacterium ulcerans in clinical samples and isolates from Ghana
American Journal of Tropical Medicine and Hygiene, Volume 83, No. 5, Year 2010
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Description
Standardized antimycobacterial therapy is considered the treatment of choice for Buruli ulcer disease. To assess the prevalence of drug resistance among clinical Mycobacterium ulcerans isolates in Ghana, we conducted a sequence-based approach to detect mutations associated with drug resistance. We subjected clinical samples to direct DNA sequencing of rpoB and rpsL genes and compared culture and whole-genome extracts regarding the efficiency of sequence analysis; 99.1% (rpoB) and 100% (rpsL) of the patients harbored M. ulcerans wild type. In one isolate (0.9%), a point mutation of the rpoB gene at codon Ser522 leading to an amino acid change was detected. Culture extracts yielded a significantly higher sequencing efficiency than whole-genome extracts. Our data suggest a low level of drug resistance in Ghana. However, mutations associated with drug resistance do occur and require monitoring. Improved techniques are necessary to enhance the efficiency of sequence analysis of whole-genome extracts. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Beißner, Marcus
Unknown Affiliation
Awua-Boateng, N. Y.
Unknown Affiliation
Thompson, William A.
Unknown Affiliation
Nienhuis, W. A.
Unknown Affiliation
Klutse, Erasmus Y.
Unknown Affiliation
Agbenorku, Pius T.
Unknown Affiliation
Nitschke, Jörg
Unknown Affiliation
Herbinger, Karl Heinz
Unknown Affiliation
Siegmund, Vera
Unknown Affiliation
Fleischmann, Erna
Unknown Affiliation
Adjei, Ohene
Unknown Affiliation
Fleischer, Bernhard
Unknown Affiliation
Van Derwerf, Tjip S.
Unknown Affiliation
Los̈cher, Thomas
Unknown Affiliation
Bretzel, Gisela
Unknown Affiliation
Statistics
Citations: 25
Authors: 15
Affiliations: 8
Identifiers
Doi:
10.4269/ajtmh.2010.10-0263
ISSN:
00029637
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study
Study Locations
Ghana