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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
PLoS ONE, Volume 12, No. 10, Article e0186079, Year 2017
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Description
Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research. © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Authors & Co-Authors
Li, Lin
Canada, Winnipeg
University of Manitoba
Omange, Robert Were
Canada, Winnipeg
University of Manitoba
Soule, Geoff
Canada, Ottawa
Public Health Agency of Canada
Kashem, Mohammad Abul
Canada, Winnipeg
University of Manitoba
Liang, Binhua B.
Canada, Ottawa
Public Health Agency of Canada
Canada, Winnipeg
University of Manitoba
Alonso Fernández, María José
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Whitney, James B.
United States, Boston
Beth Israel Deaconess Medical Center
United States, Cambridge
Massachusetts Institute of Technology
Plummer, Francis Allan
Canada, Winnipeg
University of Manitoba
Canada, Ottawa
Public Health Agency of Canada
Luo, Ma
Canada, Winnipeg
University of Manitoba
Canada, Ottawa
Public Health Agency of Canada
Statistics
Citations: 16
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0186079
ISSN:
19326203
Research Areas
Health System And Policy
Infectious Diseases
Maternal And Child Health
Participants Gender
Female