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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Performance of real-time polymerase chain reaction assays for the detection of 20 gastrointestinal parasites in clinical samples from Senegal
American Journal of Tropical Medicine and Hygiene, Volume 97, No. 1, Year 2017
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Description
Gastrointestinal parasite infections represent one of the biggest public health problems in the world. Therefore, appropriate innovative tools are needed for assessing interventions to control these infections. This study aims to compare the performance of real-time polymerase chain reaction (PCR) assays to microscopic examination for detection of intestinal parasites. A direct microscopic examination and stool concentration was performed on 98 stool samples from patients attending Senegalese hospitals. Negative microscopic control samples were also collected in Nice and Marseille (France). Species-specific primers/probes were used to detect 20commongastrointestinal protozoans and helminths. Positive frequency and the sensitivity of each real-time PCR assay were compared with conventional microscopic examination. Real-time PCR was positive in 72 of 98 samples (73.5%), whereas microscopic examination was positive in 37 (37.7%) samples (P < 0.001). The real-time PCR assays were more sensitive than microscopy, with 57.4% (31/54) versus 18.5% (10/54), respectively, in the detection of parasites in asymptomatic patients (P < 0.05). In terms of polyparasitism, there were more coinfections detected by real-time PCR assays compared with microscopic methods (25.5% versus 3.06%). In comparison to parasite prevalence on individual samples, the results showed a perfect agreement (100%) between the two techniques for seven species, whereas discrepancies were observed for the others (agreement percentage varying from 64.2% to 98.9%). Real-time PCR appeared to be superior to microscopic examination for the detection of parasites in stool samples. This assay will be useful in diagnostic laboratories and in the field for evaluating the efficacy of mass drug administration programs. © 2017 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Sow, Doudou Diagne
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
France, Marseille
Aix Marseille Université
Parola, Philippe
France, Marseille
Aix Marseille Université
Sylla, Khadime
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Ndiaye, Magatte N.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Delaunay, Pascal
France, Nice
Centre Hospitalier Universitaire de Nice, Hôpital L'archet
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Halfon, Philippe
France, Marseille
Laboratoire Alphabio
Camiade, Sabine
France, Marseille
Laboratoire Alphabio
Dieng, Thérèse H.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Tine, Roger C.K.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Faye, Babacar Félix
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Ndiaye, Jean Louis Abdourahim
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Dièng, Yémou D.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Gaye, Oumar H.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Raoult, Didier A.
France, Marseille
Aix Marseille Université
Bittar, Fadi
France, Marseille
Aix Marseille Université
Statistics
Citations: 25
Authors: 15
Affiliations: 5
Identifiers
Doi:
10.4269/ajtmh.16-0781
ISSN:
00029637
Research Areas
Health System And Policy
Study Design
Cross Sectional Study
Study Locations
Senegal