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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Insights into maize LEA proteins: From proteomics to functional approaches
Plant and Cell Physiology, Volume 53, No. 2, Year 2012
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Description
LEA (late embryogenesis abundant) proteins participate in plant stress tolerance responses, but the mechanisms by which protection occurs are not fully understood. In the present work the unfolded proteins from maize dry embryos were analyzed by mass spectrometry. Twenty embryo proteins were identified, and among them 13 corresponded to LEA-type proteins. We selected three major LEA proteins, Emb564, Rab17 and Mlg3, belonging to groups 1, 2 and 3, respectively, and we undertook a comparative study in order to highlight differences among them. The post-translational modifications of native proteins were analyzed and the anti-aggregation properties of recombinant Emb564, Rab17 and Mgl3 proteins were evaluated in vitro. In addition, the protective effects of the LEA proteins were assessed in living cells under stress in Escherichia coli cells and in Nicotiana bentamiana leaves agroinfiltrated with fluorescent LEA-green fluorescent protein (GFP) fusions. Protein visualization by confocal microscopy indicated that cells expressing Mg3-GFP showed reduced cell shrinkage effects during dehydration and that Rab17-GFP co-localized to leaf oil bodies after heat shock. Overall, the results highlight differences and suggest functional diversity among maize LEA groups. © 2011 The Author.
Authors & Co-Authors
Amara, Imen
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Odena, Antonia
Spain, Barcelona
Universitat de Barcelona
de Oliveira, Eliandre
Spain, Barcelona
Universitat de Barcelona
Moreno, Alicia
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Spain, Pozuelo de Alarcon
Csic-upm-inia-cbgp - Centro de Biotecnología y Genómica de Plantas
Masmoudi, Khaled
Tunisia, Sfax
University of Sfax
Pags, Montserrat
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Goday, Adela
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Statistics
Citations: 78
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1093/pcp/pcr183
ISSN:
00320781
e-ISSN:
14719053