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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterization of a novel vip3-type gene from Bacillus thuringiensis and evidence of its presence on a large plasmid
FEMS Microbiology Letters, Volume 244, No. 2, Year 2005
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Description
A novel vip3-type gene named vip3LB has been isolated from Bacillus thuringiensis strain BUPM95. The corresponding secreted vegetative insecticidal protein was active against the lepidopteran insect Ephestia kuehniella. The vip3LB gene was shown, for the first time, to be carried by the large plasmid containing the cry1Ia genes of B. thuringiensis. The nucleotide sequence predicted a protein of 789 amino acids residues with a calculated molecular mass of 88.5 kDa. Both nucleotide and amino acid sequences similarity analysis revealed that vip3LB is a new vip3-type gene, presenting several differences with the other vip3-type genes. Heterologous expression of the vip3LB under the control of the strong promoter PBAD was performed in Escherichia coli and the produced protein conferred insecticidal activity against Ephestia kuehniella. This novel vegetative insecticidal protein Vip3LB could be a very useful biological control agent. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Authors & Co-Authors
Mesrati, Lobna Abdelkefi
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Tounsi, Slim
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Jaoua, Samir
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Statistics
Citations: 69
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/j.femsle.2005.02.007
ISSN:
03781097
Research Areas
Genetics And Genomics