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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle
Clinical and Vaccine Immunology, Volume 15, No. 9, Year 2008
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Description
A competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.7%. Increasing the threshold to 21% increased the specificity to 100% but modestly decreased the sensitivity to 87.2%. By using 21% inhibition, the positive predictive values ranged from 90.7% (10% prevalence) to 100% (95% prevalence) and the negative predictive values ranged from 97.0% (10% prevalence) to 48.2% (95% prevalence). The assay was able to detect serum antibody as early as 7 days after intravenous inoculation. The cELISA was distributed to five different laboratories along with a reference set of 100 defined bovine serum samples, including known positive, known negative, and field samples. The pairwise concordance among the five laboratories ranged from 100% to 97%, and all kappa values were above 0.8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Goff, Will L.
United States, Washington, D.c.
Usda Agricultural Research Service
United States, Pullman
Washington State University Pullman
Johnson, Wendell Carl
United States, Washington, D.c.
Usda Agricultural Research Service
Molloy, John B.
Australia, Brisbane
Queensland Department of Agriculture and Fisheries
Jorgensen, Wayne K.
Australia, Brisbane
Queensland Department of Agriculture and Fisheries
Waldron, Susan J.
Australia, Wacol
Tick Fever Research Centre
Figueroa-Millán, Julio V.
Mexico, Santa Catarina
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias
Matthee, Olivier
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Adams, D. Scott
United States, Pullman
Vmrd, Inc.
McGuire, Travis C.
United States, Pullman
Washington State University Pullman
Pino, Ignacio
Puerto Rico
Veterinary Clinic
Mosqueda, Juan Joel
Mexico, Santa Catarina
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias
Palmer, Guy H.
United States, Pullman
Washington State University Pullman
Suárez, Carlos E.
United States, Washington, D.c.
Usda Agricultural Research Service
Knowles, Donald P.
United States, Washington, D.c.
Usda Agricultural Research Service
McElwain, Terry F.
United States, Pullman
Washington State University Pullman
United States, Pullman
Washington Animal Disease Diagnostic Laboratoiy
Statistics
Citations: 38
Authors: 15
Affiliations: 9
Identifiers
Doi:
10.1128/CVI.00150-08
ISSN:
15566811
e-ISSN:
1556679X
Study Design
Cross Sectional Study