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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Close phylogenetic relationship between Angolan and Romanian HIV-1 subtype F1 isolates
Retrovirology, Volume 6, Article 39, Year 2009
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Description
Background: Here, we investigated the phylogenetic relationships of the HIV-1 subtype F1 circulating in Angola with subtype F1 strains sampled worldwide and reconstructed the evolutionary history of this subtype in Central Africa. Methods: Forty-six HIV-1-positive samples were collected in Angola in 2006 and subtyped at the env-gp41 region. Partial env-gp120 and pol-RT sequences and near full-length genomes from those env-gp41 subtype F1 samples were further generated. Phylogenetic analyses of partial and full-length subtype F1 strains isolated worldwide were carried out. The onset date of the subtype F1 epidemic in Central Africa was estimated using a Bayesian Markov chain Monte Carlo approach. Results: Nine Angolan samples were classified as subtype F1 based on the analysis of the env-gp41 region. All nine Angolan sequences were also classified as subtype F1 in both env-gp120 and pol-RT genomic regions, and near full-length genome analysis of four of these samples confirmed their classification as "pure" subtype F1. Phylogenetic analyses of subtype F1 strains isolated worldwide revealed that isolates from the Democratic Republic of Congo (DRC) were the earliest branching lineages within the subtype F1 phylogeny. Most strains from Angola segregated in a monophyletic group together with Romanian sequences; whereas South American F1 sequences emerged as an independent cluster. The origin of the subtype F1 epidemic in Central African was estimated at 1958 (1934-1971). Conclusion: "Pure" subtype F1 strains are common in Angola and seem to be the result of a single founder event. Subtype F1 sequences from Angola are closely related to those described in Romania, and only distantly related to the subtype F1 lineage circulating in South America. Original diversification of subtype F1 probably occurred within the DRC around the late 1950s. © 2009 Guimarães et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Guimarães, Monick Lindenmeyer
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Vicente, Ana Carolina Paulo
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Otsuki, Koko
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
da Silva, Rosa Ferreira F.C.
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Angola, Luanda
Instituto Nacional de Saúde Pública
Francisco, Moises
Angola, Luanda
Instituto Nacional de Saúde Pública
da Silva, Filomena Gomes
Angola, Luanda
Instituto Nacional de Saúde Pública
Serrano, Ducelina
Angola, Luanda
Instituto Nacional de Luta Contra Sida
Morgado, Mariza Gonçalves
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Bello, Gonzalo
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Statistics
Citations: 37
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1186/1742-4690-6-39
Research Areas
Infectious Diseases
Study Locations
Angola
Congo