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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Differential Plasmodium falciparum infection of Anopheles gambiae s.s. molecular and chromosomal forms in Mali
Malaria Journal, Volume 11, Article 133, Year 2012
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Description
Background: Anopheles gambiae sensu stricto (s.s.) is a primary vector of Plasmodium falciparum in sub-Saharan Africa. Although some physiological differences among molecular and chromosomal forms of this species have been demonstrated, the relative susceptibility to malaria parasite infection among them has not been unequivocally shown. The objective of this study was to investigate P. falciparum circumsporozoite protein infection (CSP) positivity among An. gambiae s.s. chromosomal and molecular forms. Methods: Wild An. gambiae from two sites Kela (n = 464) and Sidarebougou (n = 266) in Mali were screened for the presence of P. falciparum CSP using an enzyme-linked immunosorbent assay (ELISA). Samples were then identified to molecular form using multiple PCR diagnostics (n = 713) and chromosomal form using chromosomal karyotyping (n = 419). Results: Of 730 An. gambiae sensu lato (s.l.) mosquitoes, 89 (12.2%) were CSP ELISA positive. The percentage of positive mosquitoes varied by site: 52 (11.2%) in Kela and 37 (13.9%) in Sidarebougou. Eighty-seven of the positive mosquitoes were identified to molecular form and they consisted of nine Anopheles arabiensis (21.4%), 46 S (10.9%), 31 M (12.8%), and one MS hybrid (14.3%). Sixty of the positive mosquitoes were identified to chromosomal form and they consisted of five An. arabiensis (20.0%), 21 Savanna (15.1%), 21 Mopti (30.4%), 11 Bamako (9.2%), and two hybrids (20.0%). Discussion: In this collection, the prevalence of P. falciparum infection in the M form was equivalent to infection in the S form (no molecular form differential infection). There was a significant differential infection by chromosomal form such that, P. falciparum infection was more prevalent in the Mopti chromosomal forms than in the Bamako or Savanna forms; the Mopti form was also the most underrepresented in the collection. Continued research on the differential P. falciparum infection of An. gambiae s.s. chromosomal and molecular forms may suggest that Plasmodium - An. gambiae interactions play a role in malaria transmission. © 2012 Trout Fryxell et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Fryxell, Rebecca T.Trout
United States, Knoxville
The University of Tennessee, Knoxville
Nieman, Catelyn C.
United States, Davis
University of California, Davis
Fofana, Abdrahamane S.
Mali, Bamako
University of Bamako
Lee, Yoosook
United States, Davis
University of California, Davis
Traoré, Sékou Fantamady
Mali, Bamako
University of Bamako
Cornel, Anthony John
United States, Davis
University of California, Davis
Luckhart, Shirley
United States, Sacramento
Uc Davis School of Medicine
Lanzaro, Gregory C.
United States, Davis
University of California, Davis
Statistics
Citations: 8
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1186/1475-2875-11-133
e-ISSN:
14752875
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Mali