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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
neuroscience
Target muscles for retrograde gene delivery to specific spinal cord segments
Neuroscience Letters, Volume 435, No. 1, Year 2008
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Description
Targeted retrograde gene delivery into the injured spinal cord is less invasive for the damaged tissue. One of the advantages of this approach is the possible selection of target organs according to the level of spinal cord injury. We evaluated nine candidate target organs for retrograde delivery of an adenovirus vector carrying β-galactosidase (AdV-LacZ) gene to cervical, thoracic and lumbar spinal cord segments. One week after vector injection into each muscle, we assessed the LacZ gene expression in the spinal cord by X-gal staining. The most appropriate target organs with high transduction efficacy were the sternomastoid and clavotrapezius muscles for cervical spinal cord, tibialis anterior and the gastrocnemius muscles for the lumbar spinal cord. Retrograde gene delivery to the thoracic spinal cord was inefficient probably due to the small number of anterior horn neurons in the region. Gene expression was mainly identified over the anatomical area of innervation and not into other body organs. Our results suggested that retrograde delivery of adenovirus genome to the cervical and lumbar spinal cord segments seems feasible by injection of an adenoviral vector into the appropriate target organ. Adenovirus vector is an efficient retrograde tracer since it can deliver the carried gene to a wide area of the spinal cord and not to other body organs. © 2008 Elsevier Ireland Ltd. All rights reserved.
Authors & Co-Authors
Nakajima, Hideaki
Unknown Affiliation
Uchida, Kenzou
Unknown Affiliation
Kobayashi, Shigeru
Unknown Affiliation
Inukai, Tomoo
Unknown Affiliation
Yayama, Takafumi
Unknown Affiliation
Sato, Ryuichiro
Unknown Affiliation
Mwaka, Erisa Sabakaki
Unknown Affiliation
Baba, Hisatoshi
Unknown Affiliation
Statistics
Citations: 12
Authors: 8
Identifiers
Doi:
10.1016/j.neulet.2008.01.045
ISSN:
03043940
Research Areas
Genetics And Genomics
Violence And Injury