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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations
PLoS ONE, Volume 9, No. 2, Article e87789, Year 2014
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Description
The industrial production and commercial applications of titanium dioxide nanoparticles have increased considerably in recent times, which has increased the probability of environmental contamination with these agents and their adverse effects on living systems. This study was designed to assess the genotoxicity potential of TiO2 NPs at high exposure concentrations, its bio-uptake, and the oxidative stress it generated, a recognised cause of genotoxicity. Allium cepa root tips were treated with TiO2 NP dispersions at four different concentrations (12.5, 25, 50, 100 μg/mL). A dose dependant decrease in the mitotic index (69 to 21) and an increase in the number of distinctive chromosomal aberrations were observed. Optical, fluorescence and confocal laser scanning microscopy revealed chromosomal aberrations, including chromosomal breaks and sticky, multipolar, and laggard chromosomes, and micronucleus formation. The chromosomal aberrations and DNA damage were also validated by the comet assay. The bio-uptake of TiO2 in particulate form was the key cause of reactive oxygen species generation, which in turn was probably the cause of the DNA aberrations and genotoxicity observed in this study. © 2014 Pakrashi et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3913665/bin/pone.0087789.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3913665/bin/pone.0087789.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3913665/bin/pone.0087789.s003.tif
Authors & Co-Authors
Pakrashi, Sunandan
India, Vellore
Vellore Institute of Technology
Jain, Nitin
India, Vellore
Vellore Institute of Technology
Dalai, Swayamprava
India, Vellore
Vellore Institute of Technology
Jayakumar, Jerobin
India, Vellore
Vellore Institute of Technology
Prathna, T. C.
India, Bengaluru
Indian Institute of Science
Raichur, Ashok M.
India, Bengaluru
Indian Institute of Science
South Africa, Johannesburg
University of Johannesburg
Natarajan, Chandrasekaran
India, Vellore
Vellore Institute of Technology
Mukherjee, Amitava
India, Vellore
Vellore Institute of Technology
Statistics
Citations: 149
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0087789
e-ISSN:
19326203
Research Areas
Genetics And Genomics