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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas
Plant Biotechnology Journal, Volume 9, No. 5, Year 2011
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Description
Chloroplast transformation in microalgae offers great promise for the production of proteins of pharmaceutical interest or for the development of novel biofuels. For many applications, high level expression of transgenes is desirable. We have transformed the chloroplast of Chlamydomonas reinhardtii with two genes, acrV and vapA, which encode antigens from the fish pathogen Aeromonas salmonicida. The promoters and 5' untranslated regions of four chloroplast genes were compared for their ability to drive expression of the bacterial genes. The highest levels of expression were obtained when they were placed under the control of the cis-acting elements from the psaA-exon1 gene. The expression of these chimeric genes was further increased when a nuclear mutation that affects a factor involved in psaA splicing was introduced in the genetic background of the chloroplast transformants. Accumulation of both the chimeric mRNAs and the recombinant proteins was dramatically increased, indicating that negative feedback loops limit the expression of chloroplast transgenes. Our results demonstrate the potential of manipulating anterograde signalling to alter negative regulatory feedback loops in the chloroplast and improve transgene expression. © 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
Authors & Co-Authors
Michelet, Laure
Switzerland, Geneva
Université de Genève
France, Gif-sur-yvette
Institut de Biologie et de Technologies de Saclay
Lefebvre-Legendre, Linnka
Switzerland, Geneva
Université de Genève
Burr, Sarah
Switzerland, Bern
University of Bern
Gambia, Banjul
Medical Research Council Laboratories Gambia
Rochaix, Jean David
Switzerland, Geneva
Université de Genève
Goldschmidt-Clermont, Michel
Switzerland, Geneva
Université de Genève
Statistics
Citations: 105
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1467-7652.2010.00564.x
ISSN:
14677644
e-ISSN:
14677652
Research Areas
Cancer
Genetics And Genomics