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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Mammalian Wnt3a is released on lipoprotein particles
Traffic, Volume 10, No. 3, Year 2009
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Description
Little is known about the release and intercellular transport of Wnt proteins from mammalian cells. Lipoproteins may act as carriers for the intercellular movement and gradient formation of the lipid-linked morphogens Wingless and Hedgehog in Drosophila. To investigate whether such a mechanism can occur in mammals, we have studied Wnt release in cultured mammalian cells. Wnt3a associated with lipoproteins in the culture medium and not with extracellular vesicles or exosomes. Although Wnt3a was associated with both high-density lipoproteins (HDL) and low-density lipoproteins, only HDL allowed Wnt3a release from mouse fibroblasts. Remarkably, Wnt3a lacking its palmitate moiety was released in a lipoprotein-independent manner, demonstrating the dual role of palmitoylation in membrane and lipoprotein binding. We additionally found that Wnt3a can be released from enterocyte cell lines on endogenously expressed lipoproteins. We further discuss the physiological implications of our findings. © 2009 The Authors Journal compilation © 2009 Blackwell Munksgaard.
Authors & Co-Authors
van der Westhuyzen, Deneys R.
United States, Lexington
University of Kentucky
Schmitz, Gerd
Germany, Regensburg
Universität Regensburg
Sprong, Hein
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Statistics
Citations: 88
Authors: 3
Affiliations: 6
Identifiers
Doi:
10.1111/j.1600-0854.2008.00872.x
ISSN:
16000854