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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients
Journal of Virological Methods, Volume 119, No. 2, Year 2004
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Description
Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of the HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE NS5b HCV). The overall results were compared to those obtained with another assay of the same company based on sequencing of the 5′ non-coding region (TRUGENE HCV 5′NC genotyping kit). All strains could be typed by the 5′NC typing kit, but only 62 (91; 2%) by the NS5b prototype assay. All the 62 strains typed by both methods exhibited the same pattern at the type level: 57 were type 1, 3 were type 2, and 2 were type 4. At the subtype level, eight strains that gave undetermined results by the 5′NC kit were successfully typed by the NS5b kit; eight additional strains exhibited discrepant results. The overall agreement between the two assays was 74.2% at the subtype level. In conclusion, the NS5b region appears to be much more accurate than the 5′NC region to subtype HCV strains, especially in those isolated from patients attending haemodialysis centres where the subtype distribution suggests frequent nosocomial transmissions. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Othman, Samia Ben
France, Saint-etienne
Hôpital Nord Chu de Sainte-etienne
Tunisia, Sousse
Hopital Farhat Hached Sousse
Trabelsi, Abdelhalim
Tunisia, Sousse
Hopital Farhat Hached Sousse
Monnet, A.
Unknown Affiliation
Bouzgarrou, Nadia
France, Saint-etienne
Hôpital Nord Chu de Sainte-etienne
Tunisia, Sousse
Hopital Farhat Hached Sousse
Grattard, Florence
France, Saint-etienne
Hôpital Nord Chu de Sainte-etienne
Beyou, A.
Unknown Affiliation
Bourlet, Thomas
France, Saint-etienne
Hôpital Nord Chu de Sainte-etienne
Pozzetto, Bruno
France, Saint-etienne
Hôpital Nord Chu de Sainte-etienne
Statistics
Citations: 27
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1016/j.jviromet.2004.03.002
ISSN:
01660934
Research Areas
Health System And Policy
Infectious Diseases