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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay
Journal of Virological Methods, Volume 157, No. 2, Year 2009
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Description
A rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the pol-integrase gene was developed to detect human immunodeficiency virus type 1 (HIV-1) group M. This HIV-1 RT-LAMP assay is simple and rapid, and amplification can be completed within 35 min under isothermal conditions at 60 °C. The 100% detection limit of HIV-1 RT-LAMP was determined using a standard strain (WHO HIV-1 [97/656]) in octuplicate and found to be 120 copies/ml. The RT-LAMP assay was evaluated for use for clinical diagnosis using plasma samples collected from 57 HIV-1-infected and 40 uninfected individuals in Cameroon, where highly divergent HIV-1 strains are prevalent. Of the 57 samples from infected individuals, 56 harbored group-M HIV-1 strains, such as subtypes A, B, G, F2, and circulating recombinant forms (CRFs) _01, _02, _09, _11, _13; all were RT-LAMP positive. One sample harboring group-O HIV-1 and the 40 HIV-1-uninfected samples were RT-LAMP negative. These findings indicate that HIV-1 RT-LAMP can detect HIV-1 group-M RNA from plasma samples rapidly and with high sensitivity and specificity. These data also suggest that this RT-LAMP assay can be useful for confirming HIV diagnosis, particularly in resource-limited settings. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hosaka, Norimitsu
Japan, Kanazawa
Kanazawa University
Japan, Tokyo
Eiken Chemical Co, Ltd
Ndembi, Nicaise N.
Japan, Kanazawa
Kanazawa University
Cameroon, Yaounde
Université de Yaoundé I
Ishizaki, Azumi
Japan, Kanazawa
Kanazawa University
Kageyama, Seiji
Japan, Kanazawa
Kanazawa University
Numazaki, Kei
Japan, Nasushiobara
International University of Health and Welfare Hospital
Ichimura, Hiroshi
Japan, Kanazawa
Kanazawa University
Statistics
Citations: 30
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/j.jviromet.2009.01.004
ISSN:
01660934
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Cameroon