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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda
Journal of Acquired Immune Deficiency Syndromes, Volume 46, No. 5, Year 2007
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Description
BACKGROUND: Most HIV-infected subjects on antiretroviral therapy (ART) in resource-limited settings do not undergo virologic monitoring. There is an urgent need for cheap, accessible HIV RNA assays for early diagnosis of virologic failure. We investigated filter paper transfer (FPT) of whole blood and plasma as an alternative to standard plasma-based assays for virologic monitoring in Uganda. METHODS: Whole blood (n = 306) and plasma (n = 218) from 402 subjects established on ART were spotted onto filter paper and transported to Europe for HIV RNA extraction and quantification. These results were compared to a gold standard plasma assay in Kampala. RESULTS: Of 402 ART-treated subjects, 39 (9.7%) had viremia detectable (>500 copies/mL) by local methods. Plasma FPT showed excellent agreement with gold standard, whereas whole blood yielded a large number of false-positive viral loads. CONCLUSIONS: This is the first study to investigate the use of FPT in ART-treated subjects and demonstrates that it may provide a practical, reliable method for virologic monitoring in resource-poor settings. Plasma FPT was accurate but requires centrifuge; whole blood produced a high number of false-positive results, but these were low-level. Whole blood may be sufficiently accurate if higher HIV RNA cut-offs were used to define virologic failure. © 2007 Lippincott Williams & Wilkins, Inc.
Authors & Co-Authors
Waters, Laura Jane
United Kingdom, London
Chelsea and Westminster Hospital
United Kingdom, London
St Mary's Hospital
Kambugu, Andrew Ddungu
Uganda, Kampala
Makerere University
Tibenderana, Hilda
Uganda, Kampala
Makerere University
Meya, David B.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
John, Laurence
United Kingdom, London
Chelsea and Westminster Hospital
Uganda, Kampala
Makerere University
Mandalia, Sundhiya
United Kingdom, London
Chelsea and Westminster Hospital
Nabankema, Maggie
Uganda, Kampala
Makerere University
Namugga, Irene
Uganda, Kampala
Makerere University
Quinn, Thomas Charles
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
United States, Baltimore
Johns Hopkins School of Medicine
Gazzard, Brian George L.
United Kingdom, London
Chelsea and Westminster Hospital
Reynolds, Steven James
United Kingdom, London
Chelsea and Westminster Hospital
United States, Baltimore
Johns Hopkins School of Medicine
Nelson, Mark Richard
United Kingdom, London
Chelsea and Westminster Hospital
Statistics
Citations: 48
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1097/QAI.0b013e318159d7f4
ISSN:
15254135
Research Areas
Infectious Diseases
Study Locations
Uganda