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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Determination of vitamin A in dried human blood spots by high-performance capillary electrophoresis with laser-excited fluorescence detection
Journal of Chromatography B: Biomedical Sciences and Applications, Volume 665, No. 1, Year 1995
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Description
We have developed a high-performance capillary electrophoresis (HPCE) method to analyze the retinol (vitamin A) concentration as retinol-retinol binding protein (holo-RBP) from microvolumes of serum (5-10 μl) or one to two drops (∼20 μl) of blood collected and air-dried on blood collection filter paper. A 0.64-cm diameter disk was cut from the dried whole blood specimens and the samples were dissolved in a pretreatment buffer and filtered. Filtrate was injected onto the HPCE column for analysis. The separation was carried out in a 60 cm × 50 μm I.D. fused-silica capillary and the running voltage was 20 kV. A HeCd laser with a wavelength of 325 nm was used for excitation, and the fluorescence of the holo-RBP complex was monitored at 465 nm by a photodiode. A virtual linear relationship was obtained for the retinol concentrations between HPCE and HPLC for 28 serum samples, 19 dried venous blood samples and 9 capillary dried blood spot samples, indicating that valid measures of serum retinol can be obtained from one to two drops of capillary blood collected on filter paper. The absolute detection limit for retinol by HPCE is below 3 μg/l. The method is very useful for vitamin A level screening, especially for children and premature new-born babies. © 1985.
Authors & Co-Authors
Humphrey, Jean H.
United States, Baltimore
Johns Hopkins University
Craft, Neal E.
Unknown Affiliation
Statistics
Citations: 53
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1016/0378-4347(94)00520-F
ISSN:
03784347
Research Areas
Environmental
Maternal And Child Health