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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Isolation and characterisation of Indian Ocean ciguatoxin
Toxicon, Volume 40, No. 6, Year 2002
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Description
We report the isolation and initial characterisation of Indian Ocean ciguatoxin (I-CTX) present in toxic lipid soluble extracts isolated from ciguateric fishes collected off the Republic of Mauritius in the Indian Ocean. Following i.p. injection of this extract, mice displayed symptoms that were similar, though not identical, to those produced by Pacific and Caribbean ciguatoxins (P-CTXs and C-CTXs). Using a radiolabelled brevetoxin (PbTx) binding assay and mouse bioassay guided fractionation, I-CTX was purified by Florisil, Sephadex LH-20 and TSK HW-40S chromatography with good recovery. Isolation to purity was not possible by preparative reversed phase high-performance liquid chromatography (HPLC) due to significant losses of toxicity. However, analytical reversed phase HPLC coupled to an electrospray mass spectrometry detector identified a [M+H]+ ion at m/z 1141.58 which co-eluted with activity that displaced [3H]-PbTx binding to rat brain. This mass corresponded to C-CTX-1, but the fragmentation pattern of I-CTX showed a different ratio of pseudo molecular and product ions. I-CTX was found to elute later than P-CTX-1 but was practically indistinguishable from C-CTX-1 on reversed phase HPLC, while the TSK HW-40S column chromatography differentiated I-CTX from the later eluting C-CTX-1. Taken together, these results indicate that I-CTX is a new ciguatoxin (CTX) responsible for ciguatera caused by reef fish in the Indian Ocean. © 2002 Elsevier Science Ltd. All rights reserved.
Authors & Co-Authors
Hamilton, Brett
Australia, Brisbane
The University of Queensland
Australia, Woolloongabba
Queensland Alliance for Environmental Health Sciences
Hurbungs, Mira
Mauritius, Albion
Ministry of Fisheries
Vernoux, Jean Paul
France, Caen
Université de Caen Normandie
Jones, Alun
Australia, Brisbane
The University of Queensland
Lewis, Richard James
Australia, Brisbane
The University of Queensland
Statistics
Citations: 108
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/S0041-0101(01)00259-8
ISSN:
00410101
Research Areas
Environmental
Study Locations
Mauritius