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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Real-time polymerase chain reaction for detection of Isospora belli in stool samples
Diagnostic Microbiology and Infectious Disease, Volume 61, No. 3, Year 2008
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Description
A real-time polymerase chain reaction (PCR) assay targeting the internal transcribed spacer 2 region of the ribosomal RNA gene was developed for the detection of Isospora belli DNA in fecal samples, including an internal control to detect inhibition during the amplification process. The assay was performed on species-specific DNA controls (n = 27) and a range of positive (n = 21) and negative (n = 120) stool samples, and achieved 100% specificity and sensitivity. The simple fecal sample collection procedure, the high-throughput potential, and the possibility of quantification makes the I. belli real-time PCR assay a powerful diagnostic tool for epidemiologic studies with possibilities for extension to other helminthes and protozoa using additional molecular targets. In addition, this Isospora PCR could augment the clinical laboratory diagnosis of isosporiasis, in particular, in patients with a travel history to developing countries. © 2008 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Ten Hove, Robert Jan
Netherlands, Leiden
Leids Universitair Medisch Centrum
van Lieshout, Lisette
Netherlands, Leiden
Leids Universitair Medisch Centrum
Brienen, Eric A.T.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Perez, Maria Arantza
Malawi, Zomba
University of Malawi
Verweij, Jaco J.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Statistics
Citations: 63
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.diagmicrobio.2008.03.003
ISSN:
07328893
Research Areas
Genetics And Genomics