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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Simultaneous detection of microsatellite repeats and SNPs in the macrophage migration inhibitory factor (MIF) gene by thin-film biosensor chips and application to rural field studies
Nucleic Acids Research, Volume 33, No. 13, Year 2005
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Description
Microsatellite repeat and single nucleotide polymorphisms (SNPs) are abundant sources of genetic variation, but existing methodologies cannot simultaneously detect these variants in a facile or inexpensive way. We describe herein a thin-film biosensor chip based on an allele-discriminating oligonucleotide array that enables genotyping for both microsatellite repeats and SNPs in a single analysis. We validated this methodology for the functionally polymorphic -794 CATT5-8 repeat and -173 G/C SNP present in the promoter of the human gene for macrophage migration inhibitory factor (MIF). In a comparison of 30 samples collected at a rural hospital in Zambia, we observed a 100% concordance for both the CATT repeat and G/C SNP between the biosensor methodology and the conventional capillary electrophoresis. The biosensor chips are low in cost and once printed, they are robust and require no instrumentation for analysis. When combined with multiple displacement amplification, this methodology can be utilized in primitive settings for the genotyping of nanogram quantities of DNA present in blood, dried and stored on filter paper samples. We applied this methodology to a field study of MIF genotype in children with malaria, and provide first evidence for a potential association between MIF alleles and malaria infection. We also present data supporting significant population stratification of the low- versus high-expression forms of MIF that may bear on the role of this gene in infectious diseases. © The Author 2005. Published by Oxford University Press. All rights reserved.
Authors & Co-Authors
Zhong, Xiao Bo
United States, New Haven
Yale School of Medicine
United States, Kansas City
University of Kansas Medical Center
Leng, Lin
United States, New Haven
Yale School of Medicine
Beitin, Anna M.
United States, New Haven
Yale School of Medicine
Chen, Rui
United States, New Haven
Yale School of Medicine
McDonald, Courtney
United States, New Haven
Yale School of Medicine
Hsiao, Betty
United States, New Haven
Yale School of Medicine
Jenison, Robert D.
United States, Louisville
Thermo Electron, Corp.
Kang, Insoo
United States, New Haven
Yale School of Medicine
Park, Sung Hwan
South Korea, Seoul
The Catholic University of Korea Seoul St. Mary's Hospital
Lee, Annette T.
United States, New York
Northwell Health System
Gregersen, Peter K.
United States, New York
Northwell Health System
Thuma, Philip E.
Zambia
Macha Malaria Research Institute
United States
Macha Malaria Research Institute
Bray-Ward, Patricia
United States, New Haven
Yale School of Medicine
United States, Las Vegas
Nevada Cancer Institute
Ward, David C.
United States, New Haven
Yale School of Medicine
United States, Las Vegas
Nevada Cancer Institute
Bucala, Richard John
United States, New Haven
Yale School of Medicine
Statistics
Citations: 136
Authors: 15
Affiliations: 8
Identifiers
Doi:
10.1093/nar/gni123
ISSN:
03051048
e-ISSN:
13624962
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study
Study Locations
Zambia