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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Endocrine disrupting effects of zearalenone, alpha- and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis
Toxicology Letters, Volume 206, No. 2, Year 2011
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Description
The mycotoxin zearalenone (ZEN) is a secondary metabolite of fungi which is produced by certain species of the genus Fusarium and can occur in cereals and other plant products. Reporter gene assays incorporating natural steroid receptors and the H295R steroidogenesis assay have been implemented to assess the endocrine disrupting activity of ZEN and its metabolites α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL).α-ZOL exhibited the strongest estrogenic potency (EC50 0.022±0.001nM), slightly less potent than 17-β estradiol (EC50 0.015±0.002nM). ZEN was ~70 times less potent than α-ZOL and twice as potent as β-ZOL. Binding of progesterone to the progestagen receptor was shown to be synergistically increased in the presence of ZEN, α-ZOL or β-ZOL.ZEN, α-ZOL or β-ZOL increased production of progesterone, estradiol, testosterone and cortisol hormones in the H295R steroidogenesis assay, with peak productions at 10. μM. At 100. μM, cell viability decreased and levels of hormones were significantly reduced except for progesterone. β-ZOL increased estradiol concentrations more than α-ZOL or ZEN, with a maximum effect at 10. μM, with β-ZOL (562. ±. 59. pg/ml). >. α-ZOL (494. ±. 60. pg/ml). >. ZEN (375. ±. 43. pg/ml). The results indicate that ZEN and its metabolites can act as potential endocrine disruptors at the level of nuclear receptor signalling and by altering hormone production. © 2011 Elsevier Ireland Ltd.
Authors & Co-Authors
Frizzell, Caroline
United Kingdom, Belfast
Queen's University Belfast
Ndossi, Doreen G.
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Tanzania, Morogoro
Sokoine University of Agriculture
Verhaegen, Steven
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Dahl, Ellen
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Eriksen, Gunnar Sundstøl
Norway, Oslo
Veterinærinstituttet
Sörlie, Morten
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Ropstad, Erik
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Muller, Marc
Belgium, Liege
Université de Liège
Elliott, Christopher T.
United Kingdom, Belfast
Queen's University Belfast
Connolly, L.
United Kingdom, Belfast
Queen's University Belfast
Statistics
Citations: 197
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1016/j.toxlet.2011.07.015
ISSN:
03784274
e-ISSN:
18793169
Research Areas
Cancer
Genetics And Genomics