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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress
Plant Cell, Volume 24, No. 9, Year 2012
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Description
The functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels increased. IAR3 hydrolyzes an inactive form of auxin (indole-3-acetic acid [IAA]-alanine) and releases bioactive auxin (IAA), a central phytohormone for root development. In contrast with the wild type, iar3 mutants accumulated reduced IAA levels and did not display high osmotic stress-induced root architecture changes. Transgenic plants expressing a cleavage-resistant form of IAR3 mRNA accumulated high levels of IAR3 mRNAs and showed increased lateral root development compared with transgenic plants expressing wild-type IAR3. Expression of an inducible noncoding RNA to sequester miR167a by target mimicry led to an increase in IAR3 mRNA levels, further confirming the inverse relationship between the two partners. Sequence comparison revealed the miR167 target site on IAR3 mRNA is conserved in evolutionarily distant plant species. Finally, we showed that IAR3 is required for drought tolerance. © 2012 American Society of Plant Biologists. All rights reserved.
Authors & Co-Authors
Kinoshita, Natsuko
United States, New York
Rockefeller University
Japan, Nagoya
Nagoya University
Wang, Huan
United States, New York
Rockefeller University
Kasahara, Hiroyuki
Japan, Yokohama
Riken Center for Sustainable Resource Science
Liu, Jun
United States, New York
Rockefeller University
MacPherson, Cameron Ross
United States, New York
Rockefeller University
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Machida, Yasunori
Japan, Nagoya
Nagoya University
Kamiya, Yuji
Japan, Yokohama
Riken Center for Sustainable Resource Science
Hannah, Matthew A.
Belgium, Brussels
Bayer S.a.-n.v., Belgium
Chuaa, Nam Hai
United States, New York
Rockefeller University
Statistics
Citations: 187
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1105/tpc.112.097006
ISSN:
10404651
e-ISSN:
1532298X