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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Automated focusing in bright-field microscopy for tuberculosis detection
Journal of Microscopy, Volume 240, No. 2, Year 2010
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Description
Automated microscopy to detect Mycobacterium tuberculosis in sputum smear slides would enable laboratories in countries with a high tuberculosis burden to cope efficiently with large numbers of smears. Focusing is a core component of automated microscopy, and successful autofocusing depends on selection of an appropriate focus algorithm for a specific task. We examined autofocusing algorithms for bright-field microscopy of Ziehl-Neelsen stained sputum smears. Six focus measures, defined in the spatial domain, were examined with respect to accuracy, execution time, range, full width at half maximum of the peak and the presence of local maxima. Curve fitting around an estimate of the focal plane was found to produce good results and is therefore an acceptable strategy to reduce the number of images captured for focusing and the processing time. Vollath's F4 measure performed best for full z-stacks, with a mean difference of 0.27 μm between manually and automatically determined focal positions, whereas it is jointly ranked best with the Brenner gradient for curve fitting. © 2010 The Authors Journal compilation © 2010 The Royal Microscopical Society.
Authors & Co-Authors
Osibote, Otolorin Adelaja
South Africa, Cape Town
Mrc/uct Medical Imaging Research Unit
Dendere, Ronald
South Africa, Cape Town
Mrc/uct Medical Imaging Research Unit
Krishnan, Sriram
South Africa, Cape Town
Mrc/uct Medical Imaging Research Unit
Douglas, Tania S.
South Africa, Cape Town
Mrc/uct Medical Imaging Research Unit
Statistics
Citations: 88
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1111/j.1365-2818.2010.03389.x
e-ISSN:
13652818