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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Possible association of the Plasmodium falciparum T1526C resa2 gene mutation with severe malaria
Malaria Journal, Volume 11, Article 128, Year 2012
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Description
Background: Plasmodium falciparum exports proteins that remodel the erythrocyte membrane. One such protein, called Pf155/RESA (RESA1) contributes to parasite fitness, optimizing parasite survival during febrile episodes. Resa1 gene is a member of a small family comprising three highly related genes. Preliminary evidence led to a search for clues indicating the involvement of RESA2 protein in the pathophysiology of malaria. In the present study, cDNA sequence of resa2 gene was obtained from two different strains. The proportion of P. falciparum isolates having a non-stop T1526C mutation in resa2 gene was evaluated and the association of this genotype with severity of malaria was investigated. Methods: Resa2 cDNAs of two different strains (a patient isolate and K1 culture adapted strain) was obtained by RT-PCR and DNA sequencing was performed to confirm its gene structure. The proportion of isolates having a T1526C mutation was evaluated using a PCR-RFLP methodology on groups of severe malaria and uncomplicated patients recruited in 19911994 in Senegal and in 2009 in Benin. Results: A unique ORF with an internal translation stop was found in the patient isolate (Genbank access number : JN183870), while the K1 strain harboured the T1526C mutation (Genbank access number : JN183869) which affects the internal stop codon and restores a full length coding sequence. About 14% of isolates obtained from Senegal and Benin harboured mutant T1526C parasites. Some isolates had both wild and mutant resa alleles. The analysis excluding those mixed isolates showed that the resa2 T1526C mutation was found more frequently in severe malaria cases than in uncomplicated cases (p = 0.008). The association of the presence of the mutant allele and parasitaemia ≥4% was shown in multivariate analysis (p = 0.03) in the group of Beninese children. Conclusions: All T1526C mutant parasites theoretically have the ability to give rise to a full-length RESA2 protein. This study raises the hypothesis that the RESA2 protein could favour high-density infections. Other studies in various geographic settings and probably including more patients are now required to replicate these results and to answer the questions raised by these results. © 2012 Durand et al.; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3422168/bin/1475-2875-11-128-S1.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3422168/bin/1475-2875-11-128-S2.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3422168/bin/1475-2875-11-128-S3.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3422168/bin/1475-2875-11-128-S4.xls
Authors & Co-Authors
Durand, Rémy
France, Bobigny
Hopital Avicenne
France, Paris
Mère et Enfant Face Aux Infections Tropicales
Migot-Nabias, Florence
France, Paris
Mère et Enfant Face Aux Infections Tropicales
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Andriantsoanirina, Valérie
France, Bobigny
Hopital Avicenne
Seringe, Élise
France, Paris
Hôpital Universitaire Pitié Salpêtrière
Viwami, Firmine
Benin
Centre Detude et de Recherche Sur le Paludisme Associé la Grossesse et Lenfance Cerpage
Sagbo, Gratien G.
Benin
Centre National Hospitalier et Universitaire Hubert K. Maga
Lalya, Francis
Benin
Centre National Hospitalier et Universitaire Hubert K. Maga
Deloron, Philippe
France, Paris
Mère et Enfant Face Aux Infections Tropicales
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Mercereau-Puijalon, Odile
France, Paris
Cnrs Centre National de la Recherche Scientifique
Bonnefoy, Serge
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 10
Authors: 10
Affiliations: 8
Identifiers
Doi:
10.1186/1475-2875-11-128
e-ISSN:
14752875
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health
Study Locations
Benin
Senegal