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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Helicobacter pylori dupA is polymorphic, and its active form induces proinflammatory cytokine secretion by mononuclear cells
Journal of Infectious Diseases, Volume 202, No. 2, Year 2010
Notification
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Description
Background. Infection with Helicobacter pylori possessing a newly described virulence factor-duodenal ulcer-romoting gene A (dupA)-has been associated with duodenal ulceration and increased gastric inflammation. Methods. The dupA locus of 34 strains was sequenced. A panel of dupA mutants was generated and cocultured with human gastric epithelial cells and peripheral blood mononuclear cells; proinflammatory cytokine release was measured. IL8 expression was measured in human gastric biopsy specimens and related to the dupA and cagA status of infecting strains. Results. Most H. pylori strains had a dupA allele that was longer (1884 bp; dupA1) than previously described dupA alleles, although some had truncated versions (dupA2). Unlike the best-characterized H. pylori virulence determinant, the cag pathogenicity island (cag PaI), neither dupA type induced release of interleukin (IL)-8 from gastric epithelial cells. However, infections due to dupA-positive strains were associated with higher-level mucosal IL-8 messenger RNA expression in the human stomach than were infections due to dupA-negative strains. To explain this paradox, we found that dupA1 (but not dupA2 or the cag PaI) substantially increased H. pylori-induced IL-12p40 and IL-12p70 production from CD14+ mononuclear cells. Other T helper 1-associated cytokines were also modestly induced. Conclusion. We suggest that virulent H. pylori strains cause inflammation by stimulating epithelial cells through cag-encoded proteins and mononuclear inflammatory cells through dupA1 products. © 2010 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Hussein, Nawfal Rasheed
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
University of Nottingham
Iraq, Duhok
University of Duhok
Argent, Richard H.
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
Queen's Medical Centre
United Kingdom, Nottingham
University of Nottingham
Marx, Christian K.
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
University of Nottingham
Patel, Sapna R.
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
University of Nottingham
Robinson, Karen
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
University of Nottingham
Atherton, John Christopher
United Kingdom, Nottingham
University of Nottingham Medical School
United Kingdom, Nottingham
University of Nottingham
Statistics
Citations: 75
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1086/653587
ISSN:
00221899
Research Areas
Genetics And Genomics