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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
DNA barcoding discriminates freshwater fishes from southeastern Nigeria and provides river system-level phylogeographic resolution within some species
Mitochondrial DNA, Volume 22, No. SUPPL. 1, Year 2011
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Description
Background and aims: Fishes are the main animal protein source for human beings and play a vital role in aquatic ecosystems and food webs. Fish identification can be challenging, especially in the tropics (due to high diversity), and this is particularly true for larval forms or fragmentary remains. DNA barcoding, which uses the 5′ region of the mitochondrial cytochrome c oxidase subunit I (COI) as a target gene, is an efficient method for standardized species-level identification for biodiversity assessment and conservation, pending the establishment of reference sequence libraries.Materials and methods: In this study, fishes were collected from three rivers in southeastern Nigeria, identified morphologically, and imaged digitally. DNA was extracted, PCR-amplified, and the standard barcode region was bidirectionally sequenced for 363 individuals belonging to 70 species in 38 genera. All specimen provenance data and associated sequence information were recorded in the barcode of life data systems (BOLD; www.barcodinglife.org). Analytical tools on BOLD were used to assess the performance of barcoding to identify species.Results: Using neighbor-joining distance comparison, the average genetic distance was 60-fold higher between species than within species, as pairwise genetic distance estimates averaged 10.29% among congeners and only 0.17% among conspecifics. Despite low levels of divergence within species, we observed river system-specific haplotype partitioning within eight species (11.4% of all species).Conclusion: Our preliminary results suggest that DNA barcoding is very effective for species identification of Nigerian freshwater fishes. © 2011 Informa UK, Ltd.
Authors & Co-Authors
Nwani, Christopher Didigwu
Nigeria, Abakaliki
Ebonyi State University
Becker, Sven
Canada, Guelph
Biodiversity Institute of Ontario
Braid, Heather Elizabeth
Canada, Guelph
Biodiversity Institute of Ontario
Ude, Emmanuel F.
Nigeria, Abakaliki
Ebonyi State University
Okogwu, Okechukwu Idumah
Nigeria, Abakaliki
Ebonyi State University
Hanner, Robert Harland
Canada, Guelph
Biodiversity Institute of Ontario
Statistics
Citations: 55
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.3109/19401736.2010.536537
ISSN:
19401736
e-ISSN:
19401744
Research Areas
Food Security
Genetics And Genomics
Study Locations
Nigeria