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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Approaches for enhancement of N
2
fixation efficiency of chickpea (Cicer arietinum L.) under limiting nitrogen conditions
Plant Biotechnology Journal, Volume 12, No. 3, Year 2014
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Description
Chickpea (Cicer arietinum) is an important pulse crop in many countries in the world. The symbioses between chickpea and Mesorhizobia, which fix N2 inside the root nodules, are of particular importance for chickpea's productivity. With the aim of enhancing symbiotic efficiency in chickpea, we compared the symbiotic efficiency of C-15, Ch-191 and CP-36 strains of Mesorhizobium ciceri in association with the local elite chickpea cultivar 'Bivanij' as well as studied the mechanism underlying the improvement of N2 fixation efficiency. Our data revealed that C-15 strain manifested the most efficient N2 fixation in comparison with Ch-191 or CP-36. This finding was supported by higher plant productivity and expression levels of the nifHDK genes in C-15 nodules. Nodule specific activity was significantly higher in C-15 combination, partially as a result of higher electron allocation to N2 versus H+. Interestingly, a striking difference in nodule carbon and nitrogen composition was observed. Sucrose cleavage enzymes displayed comparatively lower activity in nodules established by either Ch-191 or CP-36. Organic acid formation, particularly that of malate, was remarkably higher in nodules induced by C-15 strain. As a result, the best symbiotic efficiency observed with C-15-induced nodules was reflected in a higher concentration of the total and several major amino metabolites, namely asparagine, glutamine, glutamate and aspartate. Collectively, our findings demonstrated that the improved efficiency in chickpea symbiotic system, established with C-15, was associated with the enhanced capacity of organic acid formation and the activities of the key enzymes connected to the nodule carbon and nitrogen metabolism. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
Authors & Co-Authors
Nasr-Esfahani, Maryam
Iran, Khorramabad
Lorestan University
Sulieman, Saad A.
Japan, Yokohama
Riken Center for Sustainable Resource Science
Sudan, Khartoum
Khartoum University
Schulze, Joachim
Germany, Gottingen
Georg-august-universität Göttingen
Yamaguchi-Shinozaki, Kazuko
Japan, Tokyo
Graduate School of Agricultural and Life Sciences the University of Tokyo
Shinozaki, Kazuo
Japan, Yokohama
Riken Center for Sustainable Resource Science
Tran, Lam Son Phan
Japan, Yokohama
Riken Center for Sustainable Resource Science
Statistics
Citations: 41
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1111/pbi.12146
ISSN:
14677644
e-ISSN:
14677652
Research Areas
Environmental