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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii
Cellular Microbiology, Volume 13, No. 11, Year 2011
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Description
The early transcribed membrane proteins (ETRAMPs) are a family of small, highly charged transmembrane proteins unique to malaria parasites. Some members of the ETRAMP family have been localized to the parasitophorous vacuole membrane that separates the intracellular parasite from the host cell and thus presumably have a role in host-parasite interactions. Although it was previously shown that two ETRAMPs are critical for rodent malaria parasite liver-stage development, the importance of most ETRAMPs during the parasite life cycle remains unknown. Here, we comprehensively identify nine new etramps in the genome of the rodent malaria parasite Plasmodium yoelii, and elucidate their conservation in other malaria parasites. etramp expression profiles are diverse throughout the parasite life cycle as measured by RT-PCR. Epitope tagging of two ETRAMPs demonstrates protein expression in blood and liver stages, and reveals differences in both their timing of expression and their subcellular localization. Gene targeting studies of each of the nine uncharacterized etramps show that two are refractory to deletion and thus likely essential for blood-stage replication. Seven etramps are not essential for any life cycle stage. Systematic characterization of the members of the ETRAMP family reveals the diversity in importance of each family member at the interface between host and parasite throughout the developmental cycle of the malaria parasite. © 2011 Blackwell Publishing Ltd.
Authors & Co-Authors
Mackellar, Drew C.
United States, Seattle
Molecular and Cellular Biology Program
United States, Seattle
Seattle Biomedical Research Institute
Vaughan, Ashley M.
United States, Seattle
Seattle Biomedical Research Institute
Aly, Ahmed S.I.
United States, Seattle
Seattle Biomedical Research Institute
Saudi Arabia, Jeddah
College of Medicine-jeddah
Deleon, Sasha
United States, Seattle
Seattle Biomedical Research Institute
Kappe, Stefan H.I.
United States, Seattle
University of Washington
United States, Seattle
Seattle Biomedical Research Institute
Statistics
Citations: 25
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1462-5822.2011.01656.x
ISSN:
14625814
e-ISSN:
14625822
Research Areas
Genetics And Genomics
Infectious Diseases
Study Approach
Systematic review