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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A 1,536-well-based kinetic HTS assay for inhibitors of Schistosoma mansoni thioredoxin glutathione reductase
Assay and Drug Development Technologies, Volume 6, No. 4, Year 2008
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Description
Schistosomiasis is a major neglected tropical disease that currently affects over 200 million people and leads to over 200,000 annual deaths. Schistosoma mansoni parasites survive in humans in part because of a set of antioxidant enzymes that continuously degrade reactive oxygen species produced by the host. A principal component of this defense system has been recently identified as thioredoxin glutathione reductase (TGR), a parasite-specific enzyme that combines the functions of two human counterparts, glutathione reductase and thioredoxin reductase, and as such this enzyme presents an attractive new target for anti-schistosomiasis drug development. Herein, we present the development of a highly miniaturized and robust screening assay for TGR. The 5-μl final volume assay is based on the Ellman reagent [5,5′-dithiobis(2-nitrobenzoic acid) (DTNB)] and utilizes a high-speed absorbance kinetic read to minimize the effect of dust, absorbance interference, and meniscus variation. This assay is further applicable to the testing of other redox enzymes that utilize DTNB as a model substrate. © 2008 Mary Ann Liebert, Inc.
Authors & Co-Authors
Lea, Wendy A.
United States, Bethesda
National Human Genome Research Institute Nhgri
Jadhav, Ajit P.
United States, Bethesda
National Human Genome Research Institute Nhgri
Rai, Ganesha
United States, Bethesda
National Human Genome Research Institute Nhgri
Sayed, Ahmed Abdel Aziz
United States, Chicago
Illinois State University
Egypt, Cairo
College of Science
Cass, Cynthia L.
United States, Chicago
Illinois State University
Inglese, J.
United States, Bethesda
National Human Genome Research Institute Nhgri
Williams, David Lee
United States, Chicago
Illinois State University
United States, Chicago
Rush University Medical Center
Austin, Christopher P.
United States, Bethesda
National Human Genome Research Institute Nhgri
Simeonov, Anton
United States, Bethesda
National Human Genome Research Institute Nhgri
Statistics
Citations: 20
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1089/adt.2008.149
ISSN:
1540658X
Research Areas
Infectious Diseases