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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Development of a new method for the quantitative analysis of the extracellular polysaccharide of Neisseria meningitidis serogroup A by use of high-performance anion-exchange chromatography with pulsed-amperometric detection
Vaccine, Volume 19, No. 15-16, Year 2001
Notification
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Description
A new method for the quantitative determination of Neisseria meningitidis group A (MenA) capsular polysaccharide (CPS) has been developed. The method is based on trifluoracetic acid (TFA) hydrolysis of the CPS (2 M at 80°C for 3 h), followed by chromatographic separation and quantification of the liberated mannosamine-6-phosphate from the area of the peak obtained using an IonPac AS11 column coupled to the sensitive pulsed amperometric detector ED40. The highly selective nature of this method circumvents the interference problems associated with the classical method based on a colorimetric assay for phosphorus. Provided that suitable hydrolysis conditions can be found, this chromatographic approach might be applicable to the quantification of other bacterial antigens containing phosphorylated sugars such as meningococcal groups H, L, X and Z, and pneumococcal serotypes 6, 10A and 19. © 2001 Elsevier Science Ltd.
Authors & Co-Authors
Ricci, Stefano
Italy, Siena
Novartis Immunobiological Research Institute
Bardotti, Angela
Italy, Siena
Novartis Immunobiological Research Institute
D'Ascenzi, Sandro
Italy, Siena
Novartis Immunobiological Research Institute
Ravenscroft, Neil
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 46
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/S0264-410X(00)00427-8
ISSN:
0264410X
Study Approach
Quantitative