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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Characterization of human rotavirus recovered from children with acute diarrhea in kinshasa, democratic republic of congo
Journal of Infectious Diseases, Volume 202, No. SUPPL. 1, Year 2010
Notification
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Description
Between July and October of 2003, 2004, and 2005, outbreaks of acute gastroenteritis occurred among children <5 years of age in Kinshasa, Democratic Republic of the Congo. Stool specimens (67 in 2003, 108 in 2004, and 116 in 2005) were collected and screened for rotaviruses using either latex agglutination (Diarlex LAA; Orion Diagnostics) or enzyme immunoassay (IDEIA; DakoCytomation). The molecular characteristics of the rotavirus strains were then determined. Group A rotavirus was detected in 195 (76%) of 258 stool specimens. Polyacrylamide gel electrophoresis was used to observe the 11 rotavirus double-stranded RNA segments in 83% of the 195 rotavirus-positive specimens. Six rotavirus group A electropherotypic patterns were noted, predominantly within the short classic pattern (111 [69%]) and the long pattern (37 [23%]). Mixed patterns were noted in the 14 remaining specimens (9%). Of the 29 samples subjected to subgrouping VP6 enzyme immunoassay, subgroup I predominated. Some of the specimens collected in 2003 (n p 26), 2004 (n p 38), and 2005 (n p 52) were analyzed by reverse-transcription polymerase chain reaction, which showed that t G8P[6] and G8P[8] strains predominated in 2003, and G1P[6] strains with short electropherotypic patterns predominated in 2004 and 2005. The emergence in Kinshasa of G8 serotypes, unusually associated with the P[6] genotype, as well as uncommon G1 rotavirus strains showing a short RNA pattern, is significant in relation to the introduction of a rotavirus vaccine and underscores the need for continued rotavirus serotype surveillance in the Democratic Republic of the Congo. © 2010 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Kabue, Jean Pierre
Unknown Affiliation
Peenze, Ina
South Africa, Sovenga
University of Limpopo
de Beer, Mariet C.
South Africa, Sovenga
University of Limpopo
Esona, Mathew Dioh
South Africa, Sovenga
University of Limpopo
Lunfungula, C.
Congo
Paediatric Hospital of Kalemlembe
Biamungu, M.
Congo
Paediatric Hospital of Kalemlembe
Simba, T. R.
Congo
Paediatric Hospital of Kalemlembe
Tamfum, Jean Jacques Muyembe
Unknown Affiliation
Steele, Andrew Duncan
South Africa, Sovenga
University of Limpopo
United States, Seattle
Path Seattle
Statistics
Citations: 18
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1086/653576
ISSN:
00221899
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Locations
Congo