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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Interleukin-1β, interleukin-6, and tumor necrosis factor-α in the cerebrospinal fluid of term infants with hypoxic-ischemic encephalopathy after postnatal treatment with magnesium sulfate
Journal of Pediatric Neurology, Volume 9, No. 3, Year 2011
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Description
Proinflammatory cytokines play a role in the pathogenesis of hypoxic-ischemic encephalopathy (HIE). We aimed: 1) to characterize the expression of interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) in the cerebrospinal fluid (CSF) of term infants with HIE, and 2) to determine the impact of a single dose of magnesium sulfate (MgSO4) on their concentrations. We analyzed the samples of CSF that were collected during a randomized controlled trial on the use of MgSO 4 in neonatal asphyxia. Two samples were obtained from each subject; one was obtained on admission and the second sample was collected at 72 hr of life. The grades of HIE in the 47 included newborns were mild (n=15), moderate (n=17), and severe (n=15). After the collection of the first CSF sample, 23 newborns received a single dose of MgSO4, whereas the other 24 newborns received an equal volume of normal saline placebo. Concentrations of IL-1β, IL-6 and TNF-α in the CSF were quantified by enzyme-linked immunosorbent assay in all CSF samples. The initial concentration of IL-1β and IL-6 correlated with the severity grades of HIE (P= 004 and P=0001, respectively) while TNF-α did not. At 72 hr, a significant decline in the concentration of TNF-α (pg/mL) was observed in samples of MgSO 4-treated infants when compared to the control group (-28 ± 90 vs. 102 ± 404, P=0.01), whereas the two treatment groups did not differ in IL-1β or IL-6. Short-term clinical outcomes correlated best with IL-1β at baseline, IL-6 at baseline and IL-6 at 72 hr. We conclude that cytokine activation, as manifested by increased IL-1β and IL-6 in the CSF, is positively correlated with the severity of HIE. Treatment with a single dose of MgSO4 was efficacious in aborting the activation of TNF-α but did not alter the expression of IL-1β or IL-6. © 2011 - IOS Press and the authors. All rights reserved.
Authors & Co-Authors
Khashaba, Mohamed Talaat
Egypt, Mansoura
Mansoura University Children's Hospital
Shouman, B.
Egypt, Mansoura
Mansoura University Children's Hospital
Hasanein, Bothina M.
Egypt, Mansoura
Mansoura University Children's Hospital
Shaltout, Ali
Egypt, Mansoura
Mansoura University Children's Hospital
Al-Marsafawy, Hala M.F.
Egypt, Mansoura
Mansoura University Children's Hospital
Abdel-Aziz, Mohamed M.
Egypt, Mansoura
Faculty of Medicine
Ahmad, Tahmina
United States, Washington, D.c.
The George Washington University
Aly, Hany Z.
United States, Washington, D.c.
The George Washington University
Statistics
Citations: 8
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.3233/JPN-2011-0501
e-ISSN:
18759041
Research Areas
Cancer
Maternal And Child Health
Study Design
Randomised Control Trial
Study Approach
Quantitative