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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions
Journal of the American Chemical Society, Volume 134, No. 24, Year 2012
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Description
With the aim of developing a new tool to investigate DNA interactions, a nucleoside analogue incorporating a 3-hydroxychromone (3HC) fluorophore as a nucleobase mimic was synthesized and incorporated into oligonucleotide chains. In comparison with existing fluorescent nucleoside analogues, this dye features exceptional environmental sensitivity switching between two well-resolved fluorescence bands. In labeled DNA, this nucleoside analogue does not alter the duplex conformation and exhibits a high fluorescence quantum yield. This probe is up to 50-fold brighter than 2-aminopurine, the fluorescent nucleoside standard. Moreover, the dual emission is highly sensitive to the polarity of the environment; thus, a strong shielding effect of the flanking bases from water was observed. With this nucleoside, the effect of a viral chaperone protein on DNA base stacking was site-selectively monitored. © 2012 American Chemical Society.
Authors & Co-Authors
Klymchenko, Andrey S.
France, Illkirch-graffenstaden
Laboratoire de Bioimagerie et Pathologies - Lbp
Guerineau, Vincent
France, Gif-sur-yvette
Institut de Chimie Des Substances Naturelles
Mély, Yves
France, Illkirch-graffenstaden
Laboratoire de Bioimagerie et Pathologies - Lbp
Benhida, Rachid
France, Nice
Université Côte D'azur
Burger, Alain C.
France, Nice
Université Côte D'azur
Statistics
Citations: 80
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1021/ja3030388
ISSN:
15205126
Research Areas
Environmental
Genetics And Genomics