Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Molecular biology of extended-spectrum β-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization
Journal of Hospital Infection, Volume 57, No. 3, Year 2004
Notification
URL copied to clipboard!
Description
Twenty-nine extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae strains (14 Klebsiella pneumoniae, 10 Escherichia coli and five Citrobacter diversus) isolated from April to July 1996 from faecal carriers in a surgical intensive care unit at the university hospital of Casablanca (Morocco) were studied. Plasmid content and DNA macrorestriction polymorphism determined by pulsed-field gel electrophoresis (PFGE) were used to compare the strains. Restriction profiles of total genomic DNAs cleaved by Xba I and compared by PFGE revealed nine, four and two clones in K. pneumoniae, E. coli and C. diversus, respectively. Plasmid profile analysis of ESBL-producing strains of K. pneumoniae showed that only seven of 14 isolates had a plasmid; four different plasmid profiles were observed. Three different plasmid profiles were observed in E. coli and two in C. diversus. Plasmids responsible for ESBL production could be transferred by conjugation to E. coli K12 J53-2 from all E. coli isolates and from four of seven K. pneumoniae. No plasmid transfer could be obtained from C. diversus strains. Restriction enzyme digests of plasmids from transconjugants (four transconjugants of K. pneumoniae and five transconjugants of E. coli) showed different patterns. In the surgical intensive care unit where the survey was conducted, the dissemination of ESBLs was due to a mix of strain spread and strain diversity rather than to plasmid dissemination. © 2004 The Hospital Infection Society. Published by Elsevier by Ltd. All rights reserved.
Authors & Co-Authors
Moustaoui, Najat
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Soukri, Abdelaziz
Morocco, Casablanca
Faculté Des Sciences Aïn Chock
El Mdaghri, Naima
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Morocco, Casablanca
Laboratoire de Microbiologie
Boudouma, Mohammed
Morocco, Casablanca
Faculté Des Sciences Ben M’sick
Benbachir, Mohamed
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Morocco, Casablanca
Laboratoire de Microbiologie
Statistics
Citations: 9
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.jhin.2004.01.007
ISSN:
01956701
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Morocco