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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mitogenic potential inducible by He:Ne laser in human lymphocytes in vitro
Journal of Photochemistry and Photobiology B: Biology, Volume 68, No. 1, Year 2002
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Description
The objective of the study was to investigate the mitogenic and genotoxic effects of He:Ne laser irradiation (632.8 nm) on human peripheral lymphocytes in vitro. We used the cytokinesis-block micronucleus assay, which incorporates cytochalasin B to inhibit cytokinesis while karyokinesis proceeds normally leading to the appearance of proliferating lymphocytes as binucleated cells. Also micronuclei will appear in cases of genotoxicologically-affected cells. Buffy coat leukocytes were exposed to 10 mW He:Ne laser at energy densities of 1, 2, 3 and 5 J/cm2. Cells were then cultured in media 199 without any supplementation for 24, 48, 72 and 96 h adding cytochalasin B 24 h before harvesting of cells. Our results showed that laser-induced lymphocytes proliferate throughout the four consecutive days post-laser irradiation. The difference in the frequency of micronuclei between pre- and post-laser irradiation indicates that a He:Ne laser at such energy densities 1, 2, 3 and 5 J/cm2 does not induce micronucleus formation. These results shed some light on the mechanism encountered by lymphocytes in the process of He:Ne laser-induced biostimulation. © 2002 Elsevier Science B.V. All rights reserved.
Authors & Co-Authors
El-Batanony, Mohamed H.
Egypt, Cairo
Faculty of Medicine
Korraa, Soheir S.
Egypt, Cairo
National Center for Radiation Research and Technology
Fekry, Osama
Egypt, Cairo
Faculty of Medicine
Statistics
Citations: 19
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1016/S1011-1344(02)00319-6
ISSN:
10111344