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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Comparison of three PCR methods for detection of helicobacter pyloriDNA and detection of cagA gene in gastric biopsy specimens
World Journal of Gastroenterology, Volume 10, No. 13, Year 2004
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Description
Aim: To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacter pylori (H pylori) infection and also to evaluate the detection of a putative virulence marker of H pylori, the cagA gene, by PCR in biopsy specimens. Methods: One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms. The PCR methods used to detect H pylori DNA directly from biopsies were the glmM, 26-kDa, ureA and then cagA was used to compare the culture technique and CLO for urease with the culture technique being used as the gold standard. Results: Thirty-five percent of the biopsies were positive for H pylori DNA using the 3 PCR methods, while 68% of these were positive for the cagA gene. Twenty-four percent of the biopsies were negative for H pylori DNA in all PCR methods screened. The remaining 41% were either positive for ureA gene only, glmM only, 26-kDa only, or ureA + glmM, ureA + 26-kDa, glmM+ 26-kDa. Out of the 35% positive biopsies, 41% and 82% were positive by culture and CLO respectively, while all negative biopsies were also negative by culture and cagA. Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis. Conclusion: This method is useful for correctly identifying infections caused by H pylori and can be easily applied in our laboratory for diagnostic purposes. Copyright © 2004 by The WJG Press.
Authors & Co-Authors
Smith, Stella I.
Nigeria, Yaba
Nigerian Institute of Medical Research
Oyedeji, Kolawole Solomon
Nigeria, Yaba
Nigerian Institute of Medical Research
Arigbabu, Anthony Olu
Nigeria, Ife
Obafemi Awolowo University Teaching Hospitals Complex
Cantet, Franck
France, Bordeaux
Laboratoire de Bacteriologie
Mègraud, Françis
France, Bordeaux
Laboratoire de Bacteriologie
Ojo, O. O.
Nigeria, Ibadan
University of Ibadan
Uwaifo, Anthony O.
Nigeria, Ibadan
University of Ibadan
Otegbayo, Jesse Abiodun
Nigeria, Ibadan
University of Ibadan
Ola, Samuel Olawale
Nigeria, Ibadan
University of Ibadan
Coker, Akintoye Olusegun
Nigeria, Lagos
University of Lagos
Statistics
Citations: 98
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.3748/wjg.v10.i13.1958
ISSN:
10079327
Research Areas
Genetics And Genomics