Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Mutations in the rrs A1401G gene and phenotypic resistance to amikacin and capreomycin in mycobacterium tuberculosis
Microbial Drug Resistance, Volume 18, No. 2, Year 2012
Notification
URL copied to clipboard!
Description
The aminoglycosides amikacin (AMK)/kanamycin (KAN) and the cyclic polypeptide capreomycin (CAP) are important injectable drugs in the treatment of multidrug-resistant tuberculosis. Cross-resistance among these drug classes occurs and information on the minimum inhibitory concentrations (MICs), above the normal wild-type distribution, may be useful in identifying isolates that are still accessible to drug treatment. Isolates from the Eastern Cape Province of South Africa were subjected to DNA sequencing of the rrs (1400-1500 region) and tlyA genes. Sequencing data were compared with (i) conventional susceptibility testing at standard critical concentrations (CCs) on Middlebrook 7H11 agar and (ii) MGIT 960-based MIC determinations to assess the presence of AMK- and CAP-resistant mutants. Isolates with an rrs A1401G mutation showed high-level resistance to AMK (>20mg/L) and decreased phenotypic susceptibility to CAP (MICs 10-15mg/L). The MICs of CAP were below the bioavailability of the drug, which suggests that it may still be effective against multi- or extensively drug resistant tuberculosis [M(X)DR-TB]. Agar-based CC testing was found to be unreliable for resistance recognition of CAP in particular. © 2012 Mary Ann Liebert, Inc.
Authors & Co-Authors
Sirgel, Frederick Adriaan
South Africa, Stellenbosch
Stellenbosch University
Klopper, Marisa
South Africa, Stellenbosch
Stellenbosch University
Warren, Robin Mark
South Africa, Stellenbosch
Stellenbosch University
Streicher, Elizabeth Maria
South Africa, Stellenbosch
Stellenbosch University
Böttger, Erik Christian
Switzerland, Zurich
Universität Zürich
Van Helden, Paul D.
South Africa, Stellenbosch
Stellenbosch University
Gey van Pittius, Nicolaas Claudius
South Africa, Stellenbosch
Stellenbosch University
Coetzee, Gerrit J.
South Africa, Johannesburg
National Health Laboratory Service
Hoosain, Ebrahim Y.
South Africa, Port Elizabeth
Nelson Mandela Bay Municipality
Chabula-Nxiweni, Mamisa
South Africa, Port Elizabeth
Nelson Mandela Bay Municipality
Hayes, Cindy
South Africa, Johannesburg
National Health Laboratory Service
Victor, Thomas Calldo
South Africa, Stellenbosch
Stellenbosch University
Trollip, André Phillip
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 68
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.1089/mdr.2011.0063
ISSN:
10766294
e-ISSN:
19318448
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Locations
South Africa