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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evidence for hepatitis E virus quasispecies
Journal of General Virology, Volume 85, No. 11, Year 2004
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Description
The genetic diversity of hepatitis E virus (HEV) has been extensively analysed during the last decade. Most sporadic and epidemic HEV strains are distributed into genotypes or groups. Nevertheless, few studies have looked at the polymorphism of HEV strains isolated from a given outbreak. A serum bank collected in Tanefdour, Algeria, during an acute hepatitis epidemic (1986-1987), retrospectively confirmed as hepatitis E, was analysed. Of the 69 serum samples collected within an 8-week period, 23 were positive for both partial ORF1 (replicase gene) and ORF2 (capsid gene) sequences. Inter- and intra-patient diversities were assessed by RFLP, and by sequencing a 448 bp sequence corresponding to ORF2. RFLP analysis distinguished three profiles: A (18/23), B (3/23) and C (2/23). Most isolates (18/23) shared 99.7-100% sequence identity and the remainder showed 1-1.3% divergence. HEV intra-patient diversity was studied using 12 isolates (seven displaying the major RFLP profile and five displaying minor RFLP profiles). For 9 of 12 isolates, additional intra-patient heterogeneity was revealed by RFLP analysis of 100 clones from each isolate and sequence diversity ranging from 0.11 to 3.4%. These data strongly support the quasispecies organization of HEV during epidemics and could explain the adaptable behaviour of the virus in the host-pathogen interrelations. © 2004 SGM.
Authors & Co-Authors
Grandadam, Marc
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Tebbal, Soraya
Algeria, Batna
Centre Hospitalo-universitaire Touhami Benflis - Batna
Caron, Mélanie
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Siriwardana, Mahinda
France, Paris
Inserm
Larouzé, Bernard
France, Paris
Inserm
Koeck, Jean Louis
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Buisson, Yves
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Enouf, Vincent V.E.
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Nicand, Élisabeth
France, Paris
Hopital D'instruction Des Armées du Val de Grace
Statistics
Citations: 64
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1099/vir.0.80248-0
ISSN:
00221317
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Locations
Algeria