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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Standardization and international multicenter validation of a pulsenet pulsed-field gel electrophoresis protocol for subtyping Shigella flexneri isolates
Foodborne Pathogens and Disease, Volume 9, No. 5, Year 2012
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Description
Shigella flexneri is one of the agents most frequently linked to diarrheal illness in developing countries and often causes outbreaks in settings with poor hygiene or sanitary conditions. Travel is one of the means by which S. flexneri can be imported into developed countries, where this pathogen is not commonly seen. A robust and discriminatory subtyping method is needed for the surveillance of S. flexneri locally and regionally, and to aid in the detection and investigation of outbreaks. The PulseNet International network utilizes standardized pulsed-field gel electrophoresis (PFGE) protocols to carry out laboratory-based surveillance of foodborne pathogens in combination with epidemiologic data. A multicenter validation was carried out in nine PulseNet laboratories located in North and South America, Europe, and Asia, and it demonstrated that a new protocol is highly robust and reproducible for subtyping of S. flexneri. This protocol, already approved for PulseNet laboratories, applies NotI and XbaI as primary and secondary restriction enzymes, respectively, under electrophoresis conditions of initial switch time of 5s to final switch time of 35s, at 6 volts/cm. © Copyright 2012, Mary Ann Liebert, Inc.
Authors & Co-Authors
Pichel, Mariana
Argentina, Buenos Aires
Instituto Nacional de Enfermedades Infecciosas
Brengi, Silvina P.
Argentina, Buenos Aires
Instituto Nacional de Enfermedades Infecciosas
Cooper, Kara L.F.
United States, Atlanta
Centers for Disease Control and Prevention
Ribot, Efrain
United States, Atlanta
Centers for Disease Control and Prevention
Al-Busaidy, S. M.
Oman, Muscat
Central Public Health Laboratory
Araya, Pamela
Chile, Santiago
Instituto de Salud Publica de Chile
Fernández, Jorge
Chile, Santiago
Instituto de Salud Publica de Chile
Vaz, Tania Ibelli
Brazil, Cerqueira Cesar
Instituto Adolfo Lutz
Kam, Kaiman
Hong Kong, Hong Kong
Public Health Laboratory Centre
Morcos, Myriam
Egypt, Cairo
U.s. Naval Medical Research Unit No. 3, Egypt
Nielsen, E. Møller
Denmark, Copenhagen
Statens Serum Institut
Nadon, Celine
Canada, Winnipeg
National Microbiology Laboratory
Pimentel, Guillermo
Egypt, Cairo
U.s. Naval Medical Research Unit No. 3, Egypt
Pérez-Gutiérrez, Enrique
Panama
Vph/panaftosa
Gerner-Smidt, Peter
United States, Atlanta
Centers for Disease Control and Prevention
Binsztein, Norma
Argentina, Buenos Aires
Instituto Nacional de Enfermedades Infecciosas
Statistics
Citations: 21
Authors: 16
Affiliations: 10
Identifiers
Doi:
10.1089/fpd.2011.1067
ISSN:
15353141
e-ISSN:
15567125
Research Areas
Infectious Diseases