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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
ELISA solid phase: Partial denaturation of coating antibody yields a more efficient solid phase
Journal of Immunological Methods, Volume 59, No. 3, Year 1983
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Description
We have examined conditions which will improve the solid phase in ELISAs for HBsAg and ferritin in human serum. Sheep and rabbit antibodies were used. It was found that pre-exposure of antibody to low pH, 3 M urea and temperatures as high as 82°C will enhance ELISA colour in these assays. Further experiments indicate that these conditions perturb the structure of the antibody molecules and results in the exposure of new hydrophobic regions. It is speculated that these more hydrophobic molecules can bind to regions on the plastic surface normally not coated by non-perturbed molecules. © 1983.
Authors & Co-Authors
Conradie, Jan D.
South Africa, Pinetown
Natal Institute of Immunology
Govender, M.
South Africa, Pinetown
Natal Institute of Immunology
Visser, Leon
South Africa, Pretoria
National Chemical Research Laboratory
Statistics
Citations: 62
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1016/0022-1759(83)90190-4
ISSN:
00221759