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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Discovery of a unique novel clade of mosquito-associated bunyaviruses
Journal of Virology, Volume 87, No. 23, Year 2013
Notification
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Description
Bunyaviruses are the largest known family of RNA viruses, infecting vertebrates, insects, and plants. Here we isolated three novel bunyaviruses from mosquitoes sampled in Côte d'Ivoire, Ghana, and Uganda. The viruses define a highly diversified monophyletic sister clade to all members of the genus Orthobunyavirus and are virtually equidistant to orthobunyaviruses and tospoviruses. Maximal amino acid identities between homologous putative proteins of the novel group and orthobunyaviruses ranged between 12 and 25%. The type isolates, tentatively named Herbert virus (HEBV), Taï virus (TAIV), and Kibale virus (KIBV), comprised genomes with L, M, and S segments of about 7.4 kb, 2.7 kb, and 1.1 kb, respectively. HEBV, TAIV, and KIBV encode the shortest bunyavirusMsegments known and did not seem to encode NSs and NSm proteins but contained an elongated L segment with an ~500-nucleotide (nt) insertion that shows no identity to other bunyaviruses. The viruses replicated to high titers in insect cells but did not replicate in vertebrate cells. The enveloped virions were 90 to 110 nm in diameter and budded at cellular membranes with morphological features typical of the Golgi complex. Viral RNA recovered from infected cells showed 5'-terminal nontemplated sequences of 9 to 22 nt, suggestive of cap snatching during mRNA synthesis, as described for other bunyaviruses. Northern blotting identified RNA species of full and reduced lengths, suggested upon analogy with other bunyaviruses to constitute antigenomic-sense cRNA and transcript mRNAs, respectively. Functional studies will be necessary to determine if this group of viruses constitutes a novel genus in the bunyavirus family. © 2013, American Society for Microbiology.
Authors & Co-Authors
Marklewitz, Marco M.
Germany, Bonn
Universität Bonn
Zirkel, Florian
Germany, Bonn
Universität Bonn
Rwego, Innocent B.
United States, Atlanta
Emory University
United States, Atlanta
Rollins School of Public Health
Uganda, Kampala
Makerere University
United States, Saint Paul
College of Veterinary Medicine
Heidemann, Hanna
Germany, Bonn
Universität Bonn
Trippner, Pascal
Germany, Bonn
Universität Bonn
Kurth, Andreas
Germany, Berlin
Robert Koch Institute
Kallies, René
Germany, Bonn
Universität Bonn
Briese, Thomas C.
United States, New York
Mailman School of Public Health
Lipkin, W. Ian
United States, New York
Mailman School of Public Health
Drosten, C.
Germany, Bonn
Universität Bonn
Gillespie, Thomas R.
United States, Atlanta
Emory University
United States, Atlanta
Rollins School of Public Health
Junglen, Sandra
Germany, Bonn
Universität Bonn
Statistics
Citations: 86
Authors: 12
Affiliations: 7
Identifiers
Doi:
10.1128/JVI.01862-13
ISSN:
0022538X
e-ISSN:
10985514
Study Locations
Ghana
Ivory Coast
Uganda