Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Distribution of ace-1
R
and resistance to carbamates and organophosphates in Anopheles gambiae s.s. populations from Côte d'Ivoire
Malaria Journal, Volume 9, No. 1, Article 167, Year 2010
Notification
URL copied to clipboard!
Description
Background. The spread of pyrethroid resistance in Anopheles gambiae s.s. is a critical issue for malaria vector control based on the use of insecticide-treated nets. Carbamates and organophosphates insecticides are regarded as alternatives or supplements to pyrethroids used in nets treatment. It is, therefore, essential to investigate on the susceptibility of pyrethroid resistant populations of An. gambiae s.s. to these alternative products. Methods. In September 2004, a cross sectional survey was conducted in six localities in Côte d'Ivoire: Toumbokro, Yamoussoukro, Toumodi in the Southern Guinea savannah, Tiassalé in semi-deciduous forest, then Nieky and Abidjan in evergreen forest area. An. gambiae populations from these localities were previously reported to be highly resistant to pyrethroids insecticides. Anopheline larvae were collected from the field and reared to adults. Resistance/susceptibility to carbamates (0.4% carbosulfan, 0.1% propoxur) and organophosphates (0.4% chlorpyrifos-methyl, 1% fenitrothion) was assessed using WHO bioassay test kits for adult mosquitoes. Then, PCR assays were run to determine the molecular forms (M) and (S), as well as phenotypes for insensitive acetylcholinesterase (AChE1) due to G119S mutation. Results. Bioassays showed carbamates (carbosulfan and propoxur) resistance in all tested populations of An. gambiae s.s. In addition, two out of the six tested populations (Toumodi and Tiassalé) were also resistant to organophosphates (mortality rates ranged from 29.5% to 93.3%). The M-form was predominant in tested samples (91.8%). M and S molecular forms were sympatric at two localities but no M/S hybrids were detected. The highest proportion of S-form (7.9% of An. gambiae identified) was in sample from Toumbokro, in the southern Guinea savannah. The G119S mutation was found in both M and S molecular forms with frequency from 30.9 to 35.2%. Conclusion. This study revealed a wide distribution of insensitive acetylcholinesterase due to the G119S mutation in both M and S molecular forms of the populations of An. gambiae s.s. tested. The low cross-resistance between carbamates and organophosphates highly suggests involvement of other resistance mechanisms such as metabolic detoxification or F290V mutation. © 2010 Alou et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Ahoua Alou, Ludovic Phamien
Cote D'ivoire, Abidjan
Institut Pierre Richet Ipr
Cote D'ivoire, Abidjan
Université de Cocody-abidjan
Koffi, Alphonsine Amanan
Cote D'ivoire, Abidjan
Institut Pierre Richet Ipr
Adja, Maurice Akré
Cote D'ivoire, Abidjan
Institut Pierre Richet Ipr
Cote D'ivoire, Abidjan
Université de Cocody-abidjan
Tia, Emmanuel
Cote D'ivoire, Abidjan
Université de Bouaké
Kouassi, Philippe
Cote D'ivoire, Abidjan
Université de Cocody-abidjan
Koné, Moussa S.
Cote D'ivoire, Abidjan
Institut Pierre Richet Ipr
Chandre, Fabrice
France, Montpellier
Ird Centre de Montpellier
Statistics
Citations: 71
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1186/1475-2875-9-167
e-ISSN:
14752875
Research Areas
Cancer
Infectious Diseases
Noncommunicable Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Guinea
Ivory Coast