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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Stereoselective HPLC assay of donepezil enantiomers with UV detection and its application to pharmacokinetics in rats
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 830, No. 1, Year 2006
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Description
This investigation describes a new precise, sensitive and accurate stereoselective HPLC method for the simultaneous determination of donepezil enantiomers in tablets and plasma with enough sensitivity to follow its pharmacokinetics in rats up to 12 h after single oral dosing. Enantiomeric resolution was achieved on a cellulose tris (3,5-dimethylphenyl carbamate) column known as Chiralcel OD, with UV detection at 268 nm, and the mobile phase consisted of n-hexane, isopropanol and triethylamine (87:12.9:0.1). Using the chromatographic conditions described, donepezil enantiomers were well resolved with mean retention times of 12.8 and 16.3 min, respectively. Linear response (r > 0.994) was observed over the range of 0.05-2 μg/ml of donepezil enantiomers, with detection limit of 20 ng/ml. The mean relative standard deviation (R.S.D.%) of the results of within-day precision and accuracy of the drug were ≤10%. There was no significant difference (p > 0.05) between inter- and intra-day studies for each enantiomers which confirmed the reproducibility of the assay method. The mean extraction efficiency was 92.6-93.2% of the enantiomers. The proposed method was found to be suitable and accurate for the quantitative determination of donepezil enantiomers in tablets. The assay method also shows good specificity to donepezil enantiomers, and it could be successfully applied to its pharmacokinetic studies and to therapeutic drug monitoring. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Radwan, Mahasen Ali
Saudi Arabia, Riyadh
College of Pharmacy
Abdine, Heba H.
Saudi Arabia, Riyadh
College of Pharmacy
Al-Quadeb, Bushra T.
Saudi Arabia, Riyadh
College of Pharmacy
Aboul-Enein, Hassan Y.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Nakashima, Ken'Ichiro
Japan, Nagasaki
Nagasaki University
Statistics
Citations: 48
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.jchromb.2005.10.031
ISSN:
15700232
Research Areas
Health System And Policy
Study Approach
Quantitative